Overall distribution of GLYT2 mRNA-containing versus GAD67 mRNA-containing neurons and colocalization of both mRNAs in midbrain, pons, and cerebellum in rats
Overall distribution of GLYT2 mRNA-containing versus GAD67 mRNA-containing neurons and colocalization of both mRNAs in midbrain, pons, and cerebellum in rats
复制标题
DOI：
10.1016/j.neures.2004.02.007
复制
发表时间：
2004-06-01
影响因子：
2.9
通讯作者：
Ezure, K
中科院分区：
文献类型：
--
作者：
Tanaka, I;Ezure, K
我们旨在阐明大鼠中脑、脑桥和小脑中甘氨酸能神经元的总体分布，使用编码甘氨酸转运蛋白2 （GLYT2）的mRNA的原位杂交技术，该技术可靠地检测甘氨酸能细胞体。我们将这种方法与编码谷氨酸脱羧酶异构体67 （GAD67）的mRNA的原位杂交相结合，首次提出了甘氨酸能神经元与gaba能神经元之间分布的全局和详细视图。除了这项单检测研究外，我们还对GLYT2 mRNA和GAD67 mRNA进行了双检测，以确定共表达这些mRNA的神经元的分布。我们已经证明，脑干和小脑的许多区域，不仅仅是以前的免疫组织化学研究已经指定的区域，涉及GLYT2和GAD67 mrna的双标记神经元。特别是当轻度标记的GLYT2 mrna阳性神经元分布在GAD67 mrna阳性神经元区域时，几乎所有GLYT2 mrna阳性神经元都是GAD67 mrna阳性。仅表达GLYT2 mRNA或GAD67 mRNA的区域或神经元组相当有限，如上丘、梯形体核和浦肯野细胞。目前的研究表明，甘氨酸和GABA在单个神经元中的共释放比以往报道的更为广泛。(C) 2004爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
We aimed to clarify the overall distribution of glycinergic neurons in the midbrain, pons, and cerebellum in rats, using in situ hybridization for mRNA encoding glycine transporter 2 (GLYT2), which reliably detects glycinergic cell bodies. We combined this method with in situ hybridization for mRNA encoding glutamic acid decarboxylase isoform 67 (GAD67), and have presented for the first time global and detailed views of the distribution of glycinergic neurons in relation to GABAergic neurons. In addition to this single-detection study, we performed double-detection of GLYT2 mRNA and GAD67 mRNA to determine the distribution of neurons co-expressing these mRNAs. We have shown that many areas of the brainstem and cerebellum, not only areas where previous immunohistochemical studies have specified, involve double-labeled neurons with GLYT2 and GAD67 mRNAs. In particular, when lightly labeled GLYT2 mRNA-positive neurons were distributed within the area of GAD67 mRNA-positive neurons, almost all such GLYT2 mRNA-positive neurons were GAD67 mRNA-positive. Areas or neuron groups expressing exclusively GLYT2 mRNA or GAD67 mRNA were rather limited, such as the superior colliculus, nucleus of the trapezoid body, and Purkinje cells. The present study suggests that the corelease of glycine and GABA from single neurons is more widespread than has been reported. (C) 2004 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.