Renin cells are precursors for multiple cell types that switch to the renin phenotype when homeostasis is threatened.
Renin cells are precursors for multiple cell types that switch to the renin phenotype when homeostasis is threatened.
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DOI：
10.1016/s1534-5807(04)00134-0
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发表时间：
2004-05
影响因子：
11.8
通讯作者：
M. S. Sequeira Lopez;E. Pentz;T. Nomasa;O. Smithies;R. Gomez
中科院分区：
文献类型：
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作者：
M. S. Sequeira Lopez;E. Pentz;T. Nomasa;O. Smithies;R. Gomez
合成肾素的细胞在调节血压和液体-电解质平衡中至关重要。成年哺乳动物受到的操纵，威胁稳态增加循环肾素通过增加的数量的肾素表达/释放细胞。我们假设，成年细胞合成肾素的能力并不是随机发生在任何细胞类型中，而是取决于细胞的谱系。为了确定表达肾素的细胞的命运，我们产生了在表达肾素的细胞中表达cre重组酶的敲入小鼠，并将它们与报告小鼠杂交。结果表明，表达肾素的细胞是多种细胞的前体，这些细胞分化为不表达肾素的细胞，如平滑肌细胞、上皮细胞、系膜细胞和肾外细胞。在肾脏中，当需要额外的激素来重建体内平衡时，这些细胞保持合成肾素的能力：明显分化的细胞的特定亚群被“保留”以通过去分化和表达肾素来响应（重复地）应激，并在危机过去时重新分化。
Renin-synthesizing cells are crucial in the regulation of blood pressure and fluid-electrolyte homeostasis. Adult mammals subjected to manipulations that threaten homeostasis increase circulating renin by increasing the number of renin-expressing/-releasing cells. We hypothesize that the ability of adult cells to synthesize renin does not occur randomly in any cell type, depending instead on the cell's lineage. To determine the fate of renin-expressing cells, we generated knockin mice expressing cre recombinase in renin-expressing cells and crossed them with reporter mice. Results show that renin-expressing cells are precursors for a variety of cells that differentiate into non-renin-expressing cells such as smooth-muscle, epithelial, mesangial, and extrarenal cells. In the kidney, these cells retain the capability to synthesize renin when additional hormone is required to reestablish homeostasis: specific subpopulations of apparently differentiated cells are "held in reserve" to respond (repeatedly) by de-differentiating and expressing renin in response to stress, and re-differentiating when the crisis passes.