Isolation and Characterization of Antimicrobial-Resistant Nontyphoidal Salmonella enterica Serovars from Imported Food Products
Isolation and Characterization of Antimicrobial-Resistant Nontyphoidal Salmonella enterica Serovars from Imported Food Products
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DOI：
10.4315/0362-028x.jfp-15-564
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发表时间：
2016-08-01
影响因子：
2
通讯作者：
Khan, Ashraf A.
中科院分区：
文献类型：
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作者：
Bae, Dongryeoul;Kweon, Ohgew;Khan, Ashraf A.
本研究的目的是确定 2011 年至 2013 年从美国进口的食品中分离出的非伤寒沙门氏菌血清型的抗菌药物耐药性并阐明其耐药机制。被抗菌药物耐药性非伤寒沙门氏菌污染的食品主要从台湾、印度尼西亚、越南和中国进口。 PCR、DNA 测序和质粒分析用于表征抗菌素耐药性决定因素。 110 株肠沙门氏菌分离株中的 23 株对各种抗菌剂类别具有耐药性，包括β-内酰胺、氨基糖苷类、苯尼考、糖肽、磺酰胺、甲氧苄啶和/或氟喹诺酮类抗菌剂。其中十二株是多重耐药菌株。在一种或多种抗菌素耐药性非伤寒沙门氏菌菌株中检测到抗菌素耐药性决定簇 bla(TEM-1').、bla(OXA-1)、tetA、tetB、tetD、dfrAl、dhfrXII、chf17、aadA1、aadA2、aadA5、olfC、qnrS 以及 gyrA 和 parC 突变。质粒谱显示 23 种抗微生物菌株中的 12 种含有不相容组 Inc14113、IncHIl、IncIl、IncN、IncW 和 IncX 的质粒。流行病学和抗菌素耐药性监测数据与从进口食品中分离出的沙门氏菌菌株中抗菌素耐药性决定因素的分子特征相结合，可以提供可用于建立或实施食品安全计划以改善公众健康的信息。
The objective of this study was to determine antimicrobial resistance and elucidate the resistance mechanism in nontyphoidal Salmonella enterica serovars isolated from food products imported into the United States from 2011 to 2013. Food products contaminated with antimicrobial-resistant nontyphoidal S. enterica were mainly imported from Taiwan, Indonesia, Vietnam, and China. PCR, DNA sequencing, and plasmid analyses were used to characterize antimicrobial resistance determinants. Twentythree of 110 S. enterica isolates were resistant to various antimicrobial classes, including P-lactam, aminoglycoside, phenicol, glycopeptide, sulfonamide, trimethoprim, and/or fluoroquinolone antimicrobial agents. Twelve of the isolates were multidrug resistant strains. Antimicrobial resistance determinants bla(TEM-1')., bla(OXA-1), tetA, tetB, tetD, dfrAl, dhfrXII, chf17, aadA1, aadA2, aadA5, olfC, qnrS, and mutations of gyrA and parC were detected in one or more antimicrobial-resistant nontyphoidal S. enterica strains. Plasmid profiles revealed that 12 of the 23 antimicrobial-resistant strains harbored plasmids with incompatibility groups Inc14113, IncHIl, IncIl, IncN, IncW, and IncX. Epidemiologic and antimicrobial resistance monitoring data combined with molecular characterization of antimicrobial resistance determinants in Salmonella strains isolated from imported food products may provide information that can be used to establish or implement food safety programs to improve public health.