Partitioning of Fungal Endophyte Assemblages in Root-Parasitic Plant Cynomorium songaricum and Its Host Nitraria tangutorum
Partitioning of Fungal Endophyte Assemblages in Root-Parasitic Plant Cynomorium songaricum and Its Host Nitraria tangutorum
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根寄生植物锁阳及其寄主白刺内生真菌群落的划分
DOI：
10.3389/fmicb.2018.00666
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发表时间：
2018-04-05
影响因子：
5.2
通讯作者：
Zhang, Gang
中科院分区：
文献类型：
--
作者：
Cui, Jin-Long;Vijayakumar, Vinod;Zhang, Gang
植物内生真菌是植物的重要组成部分，甚至被视为植物的寄主器官，影响着寄主植物的生理、生态和生长发育。然而，对锁阳与白刺根寄生关系中的微生态系统和内生真菌的功能相互作用知之甚少。本文对中国红豆杉内生真菌的分布和动态进行了客观的研究。songaricum和N.基于使用高通量测序的真菌生物群系研究，结果表明，内生真菌可能是C。songaricum及其寄主N.唐古特可能是通过吸器，木质部和韧皮部的连接在维管系统。内生真菌组成的相似性表明，C. songaricum和N. tangutorum的相似性为3.88%，显著高于C. songaricum和非寄生N.唐古特鲁姆。寄生N. tangutorum与C. songaricum对非寄生N.唐古特鲁姆。在这些群丛中，内生真菌的组成随发育阶段的进行而增加。从发芽到地上部出苗，其生长速率均呈下降趋势，可能与寄主真菌的识别和反应有关。然而，在C. songaricum与寄生和未寄生N.唐古特鲁姆。对可培养内生真菌的生物活性研究表明，镰刀菌等分离物的生物活性较强。具有促进种子萌发的能力。songaricum。本研究首次报道了中国红豆杉内生真菌特殊的生态系统。songaricum及其宿主N.唐古特鲁姆。总体而言，我们假设，更深入地了解根寄生植物和其宿主之间的内生真菌的共享，运动和作用，可能会导致找到替代方法，以帮助增加民族重要药用植物的产量。
Endophytic fungi are an integral part and even seen as host organs of plant, influencing physiology, ecology, and development of host plants. However, little is known about micro-ecosystems and functional interactions of endophytic fungi in root-parasitic interactions of Cynomorium songaricum and its host Nitraria tangutorum. Here, distribution and dynamics of endophytic fungi were objectively investigated in their associations with C. songaricum and N. tangutorum based on mycobiome studies using high-throughput sequencing. Results suggest that endophytic fungi may be exchanged between C. songaricum and its host N. tangutorum probably through haustorium, connection of xylem and phloem in the vascular system. The similarity of endophytic fungal composition between C. songaricum and parasitized N. tangutorum was 3.88% which was significantly higher than the fungal similarity of 0.10% observed between C. songaricum and non-parasitized N. tangutorum. The similarities of fungal community in parasitized N. tangutorum were much closer to C. songaricum than to the non-parasitized N. tangutorum. The composition of endophytic fungi in these associations increased in progressive developmental stages of C. songaricum from sprouting to above ground emergence, and decreased subsequently probably due to host recognition and response by fungi. However, the shared fungal operational taxonomic units (OTUs) increased among interactions of C. songaricum with parasitized and non-parasitized N. tangutorum. Studies of bioactivity on culturable endophytic fungi showed that isolates such as Fusarium spp. possess the ability to promote seed germination of C. songaricum. Our study reports for the first time the special ecological system of endophytic fungi in C. songaricum and its host N. tangutorum. Overall, we hypothesize that a deeper understanding of the sharing, movement, and role of endophytic fungi between root-parasitic plant and its host may lead to finding alternative approaches to help increase the output of ethno-pharmacologically important medicinal plants.