Polar plot representation for frequency-domain analysis of fluorescence lifetimes
Polar plot representation for frequency-domain analysis of fluorescence lifetimes
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DOI：
10.1007/s10895-005-2990-8
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发表时间：
2005-09-01
影响因子：
2.7
通讯作者：
Clegg, RM
中科院分区：
文献类型：
--
作者：
Redford, GI;Clegg, RM
我们提出了应用极坐标图分析荧光寿命数据在频域中获得。这种图形分析方法对于快速FLIM测量特别有用。从复杂的荧光信号中挑选和确定潜在的寿命分量的常用方法是在多个频率下进行测量。当不可能在一个以上的频率下进行测量时，例如快速寿命成像，极坐标图分析的特定特征产生有价值的信息，并提供参与荧光物质的诊断可视化，这些荧光物质是复杂寿命分布的基础。在极坐标图显示有助于获得有关基础成分分布的有价值的独特信息的情况下，提供数据。我们还讨论了文物的光解，以及如何这种方法也可以应用到样品中，每个荧光物种显示出连续分布的寿命。频域数据的极坐标图通常用于介电弛豫实验的分析（Cole-Cole图），几十年来，该图已被证明在该领域非常有用。我们比较了这种分析工具，这是发达国家和广泛使用的介电弛豫和化学动力学荧光测量。
We present applications of polar plots for analyzing fluorescence lifetime data acquired in the frequency domain. This graphical, analytical method is especially useful for rapid FLIM measurements. The usual method for sorting out and determining the underlying lifetime components from a complex fluorescence signal is to carry out the measurement at multiple frequencies. When it is not possible to measure at more than one frequency, such as rapid lifetime imaging, specific features of the polar plot analysis yield valuable information, and provide a diagnostic visualization of the participating fluorescent species underlying a complex lifetime distributions. Data are presented where this polar plot presentation is useful to derive valuable, unique information about the underlying component distributions. We also discuss artifacts of photolysis and how this method can also be applied to samples where each fluorescence species shows a continuous distribution of lifetimes. Polar plots of frequency-domain data are commonly used for analysis of dielectric relaxation experiments (Cole-Cole plots), which have proved to be exceptionally useful in that field for decades. We compare this analytical tool that is well developed and extensively used in dielectric relaxation and chemical kinetics to fluorescence measurements.