Construction of extracellular microenvironment to improve surface endothelialization of NiTi alloy substrate.
Construction of extracellular microenvironment to improve surface endothelialization of NiTi alloy substrate.
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DOI：
10.1016/j.msec.2015.05.047
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发表时间：
2015-10
期刊：
影响因子：
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通讯作者：
Peng Liu;Yongchun Zhao;Yingdi Yan;Yan Hu;Weihu Yang;K. Cai
中科院分区：
文献类型：
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作者：
Peng Liu;Yongchun Zhao;Yingdi Yan;Yan Hu;Weihu Yang;K. Cai
为了模拟内皮细胞的细胞外微环境，采用层层组装技术在NiTi合金表面构建了血管内皮生长因子（vascular endothelial growth factor，VEGF）包埋的明胶/壳聚糖（gelatin/chitosan）生物活性多层结构。通过扫描电子显微镜、原子力显微镜、接触角测量、衰减全反射-傅立叶变换红外光谱和X射线光电子能谱等测试手段，证实了多层膜结构的成功制备。研究了内皮细胞在不同NiTi合金基底上的体外生长行为。细胞骨架观察、MTT实验和伤口愈合实验证明，VEGF包埋的多层结构能促进内皮细胞的粘附、增殖和运动反应。更重要的是，本系统促进了内皮细胞的一氧化氮产生。该方法为构建细胞外微环境以改善心血管植入物的表面内皮化提供了一种替代方法。
To mimic extracellular microenvironment of endothelial cell, a bioactive multilayered structure of gelatin/chitosan pair, embedding with vascular endothelial growth factor (VEGF), was constructed onto NiTi alloy substrate surface via a layer-by-layer assembly technique. The successful fabrication of the multilayered structure was demonstrated by scanning electron microscopy, atomic force microscopy, contact angle measurement, attenuated total reflection-fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The growth behaviors of endothelial cells on various NiTi alloy substrates were investigated in vitro. Cytoskeleton observation, MTT assay, and wound healing assay proved that the VEGF-embedded multilayer structure positively stimulated adhesion, proliferation and motogenic responses of endothelial cells. More importantly, the present system promoted the nitric oxide production of endothelial cells. The approach affords an alternative to construct extracellular microenvironment for improving surface endothelialization of a cardiovascular implant.