DEHYDROEPIANDROSTERONE ANTAGONIZES THE SUPPRESSIVE EFFECTS OF DEXAMETHASONE ON LYMPHOCYTE-PROLIFERATION
DEHYDROEPIANDROSTERONE ANTAGONIZES THE SUPPRESSIVE EFFECTS OF DEXAMETHASONE ON LYMPHOCYTE-PROLIFERATION
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DOI：
10.1210/endo-129-6-3174
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发表时间：
1991-12-01
期刊：
影响因子：
4.8
通讯作者：
BERNTON, EW
中科院分区：
文献类型：
--
作者：
BLAUER, KL;POTH, M;BERNTON, EW
在几种动物模型中，给予脱氢表雄酮(DHEA)似乎具有与糖皮质激素相反的生理效应。最近，脱氢表雄酮治疗的免疫调节作用已被描述。本文报道了脱氢表雄酮(DHEA)对(CH)-H-3/HEN小鼠脾细胞母细胞分化反应及胸腺和脾重量的影响。小鼠皮下注射脱氢表雄酮(60 mg/kg.day)3d，可拮抗单次注射地塞米松(DEX；60 mg/kg)对T、B淋巴细胞体外母细胞反应的抑制作用。DHEA预处理也显著减轻地塞米松引起的胸腺和脾萎缩。DHEA处理的小鼠脾淋巴细胞对10(-6)-10(-8)M的DEX的体外抑制作用明显高于对照小鼠。然而，在体外淋巴细胞培养中加入浓度在10(-7)-10(-8)M范围内的DHEA，不能抵抗加入DEX引起的有丝分裂反应的抑制。综上所述，DHEA体内给药可拮抗DEX对小鼠淋巴靶组织的抑制作用。
Administration of dehydroepiandrosterone (DHEA) appears to have physiological effects opposing those of glucocorticoids in several animal models. Recently, immunomodulatory effects of treatment with DHEA have been described. This paper reports the effects of DHEA treatment on splenocyte blastogenic responses as well as thymic and spleen weights in (CH)-H-3/HeN mice. Pretreatment of mice with sc DHEA (60 mg/kg.day) for 3 days in vivo antagonized the profound suppression of in vitro blastogenic responses seen in T- and B-lymphocytes after a single injection of dexamethasone (DEX; 60 mg/kg). Pretreatment with DHEA also significantly reduced dexamethasone-induced thymic and splenic atrophy. Splenic lymphocytes from DHEA-treated mice were markedly more resistant to in vitro suppression of blastogenesis by DEX at 10(-6)-10(-8) M compared to lymphocytes from control mice. However, DHEA added to lymphocyte cultures in vitro over a concentration range from 10(-7)-10(-8) M failed to protect against suppression of mitogenic responses caused by addition of DEX to cultures. In summary, DHEA given in vivo antagonizes the suppressive actions of DEX on lymphoid target tissues in mice.