The Root-Knot Nematode Calreticulin Mi-CRT Is a Key Effector in Plant Defense Suppression
The Root-Knot Nematode Calreticulin Mi-CRT Is a Key Effector in Plant Defense Suppression
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DOI：
10.1094/mpmi-05-12-0130-r
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发表时间：
2013-01-01
影响因子：
3.5
通讯作者：
Rosso, M. N.
中科院分区：
文献类型：
--
作者：
Jaouannet, M.;Magliano, M.;Rosso, M. N.
根结线虫（RKN）是专性活体营养型寄生虫，其在根中的维管组织附近定居，在那里它们诱导特化饲养细胞的分化并维持3至8周的相容性相互作用。植物对寄生虫感染的反应的转录组分析表明，植物防御在相互作用期间受到严格控制。这表明，与其他病原体类似，RKN分泌抑制宿主防御的效应物。在这里，我们表明，Mi-CRT，钙网蛋白（CRT）分泌的线虫到受感染组织的质外质，感染成功中起着重要的作用，因为Mi-CRT敲低RNA干扰影响的线虫感染植物的能力。稳定转化的拟南芥植物产生分泌形式的Mi-CRT比野生型植物更容易受到线虫感染。它们也更容易感染另一种根病原体，卵菌寄生疫霉。Mi-CRT在A.在用病原体相关分子模式ELF 18处理后，拟南芥抑制了防御标记基因的诱导和胼胝质沉积。我们的研究结果表明，由线虫质外质中分泌的Mi-CRT在相互作用过程中抑制植物的基础防御中发挥作用。
Root-knot nematodes (RKN) are obligate biotrophic parasites that settle close to the vascular tissues in roots, where they induce the differentiation of specialized feeding cells and maintain a compatible interaction for 3 to 8 weeks. Transcriptome analyses of the plant response to parasitic infection have shown that plant defenses are strictly controlled during the interaction. This suggests that, similar to other pathogens, RKN secrete effectors that suppress host defenses. We show here that Mi-CRT, a calreticulin (CRT) secreted by the nematode into the apoplasm of infected tissues, plays an important role in infection success, because Mi-CRT knockdown by RNA interference affected the ability of the nematodes to infect plants. Stably transformed Arabidopsis thaliana plants producing the secreted form of Mi-CRT were more susceptible to nematode infection than wild-type plants. They were also more susceptible to infection with another root pathogen, the oomycete Phytophthora parasitica. Mi-CRT overexpression in A. thaliana suppressed the induction of defense marker genes and callose deposition after treatment with the pathogen-associated molecular pattern elf18. Our results show that Mi-CRT secreted in the apoplasm by the nematode has a role in the suppression of plant basal defenses during the interaction.