A polysaccharide from Andrographis paniculata induces mitochondrial-mediated apoptosis in human hepatoma cell line (HepG2)
A polysaccharide from Andrographis paniculata induces mitochondrial-mediated apoptosis in human hepatoma cell line (HepG2)
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DOI：
10.1007/s13277-015-3172-1
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
Y. Zou;Hua Xiong;Huihua Xiong;T. Lu;Feng Zhu;Zhiyong Luo;Xianglin Yuan;Yihua Wang
中科院分区：
文献类型：
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作者：
Y. Zou;Hua Xiong;Huihua Xiong;T. Lu;Feng Zhu;Zhiyong Luo;Xianglin Yuan;Yihua Wang
本文研究了穿心莲多糖（APWP）对人肝癌细胞HepG 2的作用及其机制。结果表明，APWP可通过诱导HepG 2细胞凋亡抑制HepG 2细胞增殖。Western blot分析显示，APWP处理的细胞中促凋亡Bax蛋白呈剂量依赖性增加，而抗凋亡Bcl-2蛋白无变化。此外，肿瘤细胞暴露于APWP导致线粒体膜电位（MMP）的损失和细胞色素c从线粒体释放到胞质溶胶。此外，caspase-9和caspase-3在HepG 2细胞中被激活，而caspase-8在APWP处理后没有受到影响。所有这些结果清楚地表明，APWP诱导的细胞凋亡中，APWP是安全和有效的预防和治疗肝癌的信号通路的参与。
In the present study, we investigated the effects and action mechanisms of a purified polysaccharide (APWP) fromAndrographis paniculata, on human hepatocellular carcinoma (HCC) HepG2 cells. The results showed that APWP was able to suppress the proliferation of HepG2 cells via inducing apoptosis. Western blot analysis revealed that dose-dependent increase in proapoptotic Bax protein and no change in antiapoptotic Bcl-2 protein in APWP-treated cells. Furthermore, exposure of tumor cells to APWP resulted in a loss of mitochondrial membrane potential (MMP) and the release of cytochrome c from the mitochondria to the cytosol. Besides, caspase-9 and caspase-3 were activated while caspase-8 was not affected in HepG2 cells followed by APWP treatment. All these results point clearly to the involvement of mitochondria-mediated signaling pathway in APWP-induced apoptosis and strongly suggest that APWP seems to be safe and effective in the prevention and treatment of HCC.