Photodynamic Coatings on Polymer Microfibers for Pathogen Inactivation: Effects of Application Method and Composition
Photodynamic Coatings on Polymer Microfibers for Pathogen Inactivation: Effects of Application Method and Composition
复制标题
聚合物微纤维上的光动力涂层用于灭活病原体：应用方法和成分的影响
DOI：
10.1021/acsami.0c16953
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发表时间：
2021
影响因子：
9.5
通讯作者：
Ghiladi, Reza A.
中科院分区：
文献类型：
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作者：
Peddinti, Bharadwaja S.;Morales-Gagnon, Nicolas;Pourdeyhimi, Behnam;Scholle, Frank;Spontak, Richard J.;Ghiladi, Reza A.
医院获得性感染（HAI）风险的大幅增加极大地影响了全球医疗保健行业。有害的病原体附着在各种表面上，并感染接触人员，从而促进向新宿主的传播。这在抗药性病原体的情况下尤其令人担忧，它们对全世界人类健康构成日益严重的威胁，需要新的预防性消毒途径。在这项研究中，我们已经纳入了不同的负载水平的卟啉光敏剂能够产生反应性单线态氧在O2和可见光的存在下，在水溶性，光交联聚合物涂层，随后沉积在聚合物微纤维。两种不同的应用方法被认为是，这些涂层纤维的形态和化学特性进行了分析，以检测涂层和光敏剂的存在。为了辨别纤维对致病菌的功效，对两种不同的细菌菌株，金黄色葡萄球菌和耐药性大肠杆菌进行了光动力灭活，在暴露于可见光1小时后，种群减少分别>99.9999和99.6%。为了应对当前的COVID-19大流行，我们还确认这些涂层纤维可以作为SARS-CoV-2病毒的替代品来抑制人类普通感冒冠状病毒。
A substantial increase in the risk of hospital-acquired infections (HAIs) has greatly impacted the global healthcare industry. Harmful pathogens adhere to a variety of surfaces and infect personnel on contact, thereby promoting transmission to new hosts. This is particularly worrisome in the case of antibiotic-resistant pathogens, which constitute a growing threat to human health worldwide and require new preventative routes of disinfection. In this study, we have incorporated different loading levels of a porphyrin photosensitizer capable of generating reactive singlet oxygen in the presence of O2and visible light in a water-soluble, photo-cross-linkable polymer coating, which was subsequently deposited on polymer microfibers. Two different application methods are considered, and the morphological and chemical characteristics of these coated fibers are analyzed to detect the presence of the coating and photosensitizer. To discern the efficacy of the fibers against pathogenic bacteria, photodynamic inactivation has been performed on two different bacterial strains,Staphylococcus aureusand antibiotic-resistantEscherichia coli, with population reductions of >99.9999 and 99.6%, respectively, after exposure to visible light for 1 h. In response to the current COVID-19 pandemic, we also confirm that these coated fibers can inactivate a human common cold coronavirus serving as a surrogate for the SARS-CoV-2 virus.