Annexin A2 expression and phosphorylation are up-regulated in hepatocellular carcinoma
Annexin A2 expression and phosphorylation are up-regulated in hepatocellular carcinoma
复制标题
DOI：
10.3892/ijo_00000105
复制
发表时间：
2008-12-01
影响因子：
5.2
通讯作者：
El-Sayed, Adel A.
中科院分区：
文献类型：
--
作者：
Mohammad, Hamdy Saad;Kurokohchi, Kazutaka;El-Sayed, Adel A.
膜联蛋白 (ANX) 构成 Ca2+ 依赖性膜结合蛋白家族；迄今为止，其中至少有 20 个已被描述。其中，膜联蛋白 A2 (ANXA2) 已被证明是一种体外多功能蛋白。然而，其在体内的实际作用需要进一步研究。我们已经报道 ANX-I (ANXA1) 在肝细胞癌 (HCC) 中表达上调。 ANXA2 在包括 HCC 在内的各种肝脏疾病中的作用仍不清楚。在本研究中，测定了正常人肝脏、慢性肝炎肝脏以及HCC组织的非肿瘤和肿瘤部分的ANXA2的蛋白和mRNA水平及其定位。 ANXA2 在正常或慢性肝炎肝组织中很少检测到，而在 HCC 肿瘤和非肿瘤区域的转录和翻译水平上均过表达。此外，在许多情况下，HCC的肿瘤部分比非肿瘤部分表达更多的ANXA2。 ANXA2的表达主要定位于癌细胞，尤其是低分化HCC中。此外，ANXA2 在 HCC 中被酪氨酸磷酸化。这些数据表明ANXA2的过表达和酪氨酸磷酸化在导致HCC的恶性转化过程中发挥重要作用，并且与HCC的组织学分级相关。
Annexins (ANXs) constitute it family of Ca2+-dependent membrane-binding proteins; at least 20 of them have been described to date. Among these, Annexin A2 (ANXA2) has been revealed as a multi-functional protein in vitro. Its actual role in vivo, however, requires further investigation. We already reported that ANX-I (ANXA1) was Up-regulated in hepatocellular carcinoma (HCC). The role of ANXA2 in various liver diseases including HCC remains obscure. In the present study, the protein and mRNA levels of ANXA2, as well as its localization, were determined for the normal human liver, chronic hepatitis liver, and non-tumorous and tumorous portions of HCC tissues. ANXA2 was rarely detected in either normal or chronic hepatitis liver tissues, whereas it was overexpressed at both the transcriptional and translational levels in tumorous and non-tumorous regions of HCC. In addition, in many cases, more ANXA2 was expressed in the tumorous portion than in the non-tumorous portion of HCC. The expression of ANXA2 was mainly localized in cancer cells, especially in poorly differentiated HCC. Furthermore, ANXA2 was tyrosine-phosphorylated in HCC. These data suggest that overexpression and tyrosine phosphorylation of ANXA2 play important roles in the malignant transformation process leading to HCC and are related to the histological grade of HCC.