Cellular dye lasers: lasing thresholds and sensing in a planar resonator
Cellular dye lasers: lasing thresholds and sensing in a planar resonator
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DOI：
10.1364/oe.23.027865
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发表时间：
2015-10-19
期刊：
影响因子：
3.8
通讯作者：
Yun, Seok-Hyun
中科院分区：
文献类型：
--
作者：
Humar, Matjaz;Gather, Malte C.;Yun, Seok-Hyun
生物细胞激光器是未来生物相容性光学系统的有前途的新型构建模块，并为微流体环境中的细胞传感和细胞计数提供了新的方法。在这里，我们展示了一个简单的方法，通过使用各种标准的荧光染料提供光学增益。染料增益介质可以位于细胞内部或外部，或两者中，这在实验设计中提供了灵活性，并使该方法适用于所有细胞类型。由于细胞质的折射率高于周围介质，细胞在平面法布里-珀罗腔中充当凸透镜。分析了它对激光腔稳定性的影响，并利用它来抑制细胞外的激射。共振模式取决于细胞的形状和内部结构。作为概念的证明，我们展示了激光输出模式如何受到渗透压的影响。(C)2015美国光学学会
Biological cell lasers are promising novel building blocks of future biocompatible optical systems and offer new approaches to cellular sensing and cytometry in a microfluidic setting. Here, we demonstrate a simple method for providing optical gain by using a variety of standard fluorescent dyes. The dye gain medium can be located inside or outside a cell, or in both, which gives flexibility in experimental design and makes the method applicable to all cell types. Due to the higher refractive index of the cytoplasm compared to the surrounding medium, a cell acts as a convex lens in a planar Fabry-Perot cavity. Its effect on the stability of the laser cavity is analyzed and utilized to suppress lasing outside cells. The resonance modes depend on the shape and internal structure of the cell. As proof of concept, we show how the laser output modes are affected by the osmotic pressure. (C) 2015 Optical Society of America