Heavy Metal Exposure Leads to Rapid Changes in Cellular Biophysical Properties
Heavy Metal Exposure Leads to Rapid Changes in Cellular Biophysical Properties
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DOI：
10.1021/acsbiomaterials.9b01640
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发表时间：
2020-04-01
影响因子：
5.8
通讯作者：
Sun，Yubing
中科院分区：
文献类型：
--
作者：
Zhu，Peiran;Hawkins，Jamar;Sun，Yubing
细胞的生物物理特性，如细胞力学、细胞形状和细胞迁移，是表征各种细胞功能的新兴标志。相反，这些生物物理特性的破坏可以用作细胞稳态破坏的可靠指标，例如在化学诱导毒性的情况下。在这项研究中，我们表明，硝酸铅（II）和硝酸镉的治疗导致剂量依赖性的变化，在生物物理特性的集合，包括细胞牵引力，局灶性粘连，机械刚度，细胞形状，迁移速度，渗透性，和伤口愈合在哺乳动物细胞的疗效。由于这些变化在用微量铅/镉处理后几个小时内出现，我们的研究结果突出了利用生物物理特性筛选环境化学品以识别潜在毒物并建立剂量响应曲线的前景。我们的系统和定量表征的细胞骨架结构和细胞功能的快速变化后，重金属处理可能会激发新的研究的毒性机制。
Biophysical properties of cells, such as cell mechanics, cell shape, and cell migration, are emerging hallmarks for characterizing various cell functions. Conversely, disruptions to these biophysical properties may be used as reliable indicators of disruptions to cell homeostasis, such as in the case of chemical-induced toxicity. In this study, we demonstrate that treatment of lead(II) nitrate and cadmium nitrate leads to dosage-dependent changes in a collection of biophysical properties, including cellular traction forces, focal adhesions, mechanical stiffness, cell shape, migration speed, permeability, and wound-healing efficacy in mammalian cells. As those changes appear within a few hours after the treatment with a trace amount of lead/cadmium, our results highlight the promise of using biophysical properties to screen environmental chemicals to identify potential toxicants and establish dose response curves. Our systematic and quantitative characterization of the rapid changes in cytoskeletal structure and cell functions upon heavy metal treatment may inspire new research on the mechanisms of toxicity.