A New Assay Based on Thrombin Generation Inhibition to Detect Both Protein C and Protein S Deficiencies in Plasma
A New Assay Based on Thrombin Generation Inhibition to Detect Both Protein C and Protein S Deficiencies in Plasma
复制标题
基于凝血酶生成抑制的新检测方法可检测血浆中蛋白 C 和蛋白 S 的缺乏
作者：
J. Duchemin;J. Pittet;Michel Tartary;S. Béguin;P. Gaussem;M. Alhenc‐Gelas;M. Aiach
总结活化蛋白C通过在蛋白S存在下灭活因子V和VIII减少凝血酶生成。这促使我们开发了一种可以具体探索这种反应的测定方法。通过加入血栓调节蛋白，在组织因子和磷脂活化后血浆中形成的凝血酶总量减少。这种添加允许蛋白C被内源性凝血酶激活。由于蛋白C活化而引起的凝血酶生成（ITG）的抑制可以通过比较存在和不存在血栓调节蛋白时的凝血酶形成来测量。ITG随蛋白C和蛋白S浓度的增加而增加。ITG的正常值以百分比表示，在40%和65%之间，不受年龄或性别的影响。接受肝素治疗的患者ITG增加，接受口服抗凝剂治疗的患者ITG减少，使用口服避孕药的女性ITG减少。该方法可用于筛查蛋白C和蛋白S缺乏症患者。
Summary Activated protein C reduces thrombin generation by inactivating factors V and VIII in the presence of protein S. This prompted us to develop an assay which would allow specific exploration of this reaction. The total amount of thrombin formed in plasma after activation by tissue factor and phospholipids was reduced by adding thrombomodulin. This addition allowed protein C to be activated by endogenous thrombin. The inhibition of thrombin generation (ITG) due to protein C activation could be measured by comparing thrombin formation in the presence and in the absence of thrombomodulin. ITG increased with both protein C and protein S concentrations. Normal values of ITG expressed as a percentage were between 40 and 65% and were not influenced by age or sex. ITG increased in patients under heparin therapy, decreased in patients under oral anticoagulant therapy and was decreased in women using oral contraceptives. This method could be used for screening patients for protein C and protein S deficiencies.
口服抗凝期间和选定疾病状态下蛋白 C 抗原与抗凝活性之间的关系。
DOI：
10.1172/jci112319
发表时间：
1986
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
ViganoD'Angelo,S;Comp,PC;Esmon,CT;D'Angelo,A
通讯作者：
D'Angelo,A
影响因子：
15.9
作者：
COMP, PC;NIXON, RR;ESMON, CT
通讯作者：
ESMON, CT
影响因子：
158.5
作者：
COMP, PC;ESMON, CT
通讯作者：
ESMON, CT