Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees?
Can the exposure of Apis mellifera (Hymenoptera, Apiadae) larvae to a field concentration of thiamethoxam affect newly emerged bees?
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DOI：
10.1016/j.chemosphere.2017.06.113
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发表时间：
2017-10-01
期刊：
影响因子：
8.8
通讯作者：
Roat, Thaisa Cristina
中科院分区：
文献类型：
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作者：
Friol, Priscila Sepulveda;Catae, Aline Fernanda;Roat, Thaisa Cristina
在农作物上使用杀虫剂可能会影响非目标昆虫，例如蜜蜂。除了成年蜜蜂外，幼虫也可能通过受污染的花卉资源接触到杀虫剂。因此，本研究旨在探讨蜜蜂幼虫暴露于田间浓度的噻虫嗪（0.001 ng/μL 噻虫嗪）对幼虫和蛹的存活率以及成虫羽化百分比的可能影响。此外，还分析了其对新出现的意大利蜜蜂中肠消化细胞、马氏小管细胞和大脑凯尼恩细胞的细胞毒性作用。结果表明，幼虫暴露于该浓度的噻虫嗪不会影响幼虫和蛹的存活率或成年蜜蜂的羽化百分比。然而，这种暴露导致了新出现的蜜蜂的目标和非目标器官的超微结构改变。接触杀虫剂的蜜蜂消化细胞表现出基底迷宫，没有长而细的通道，线粒体也受损。在马氏小管细胞中，观察到基底迷路紊乱、线粒体扩张且形状变形、嵴缺失、微绒毛紊乱。结果表明，暴露的蜜蜂在凯尼恩细胞中出现了细胞核和线粒体的改变。这些变化表明可能存在组织退化，证明了噻虫嗪在新出现的蜜蜂的目标和非目标器官中具有细胞毒性。这些结果表明细胞器损伤会损害中肠细胞、马氏小管细胞和凯尼恩细胞的细胞功能，从而损害整个蜂群蜜蜂的寿命。 (C) 2017 Elsevier Ltd. 保留所有权利。
The use of insecticides on crops can affect non-target insects, such as bees. In addition to the adult bees, larvae can be exposed to the insecticide through contaminated floral resources. Therefore, this study aimed to investigate the possible effects of the exposure of A. mellifera larvae to a field concentration of thiamethoxam (0.001 ng/mu L thiamethoxam) on larval and pupal survival and on the percentage of adult emergence. Additionally, its cytotoxic effects on the digestive cells of midgut, Malpighian tubules cells and Kenyon cells of the brain of newly emerged A. mellifera bees were analyzed. The results showed that larval exposure to this concentration of thiamethoxam did not influence larval and pupal survival or the percentage of adult bee emergence. However, this exposure caused ultra-structural alterations in the target and non-target organs of newly emerged bees. The digestive cell of bees that were exposed to the insecticide exhibited a basal labyrinth without long and thin channels and compromised mitochondria. In Malpighian tubules cells, disorganized basal labyrinth, dilated mitochondria with a deformed shape and a loss of cristae, and disorganized microvilli were observed. The results showed that the exposed bees presented Kenyon cells with alterations in the nucleus and mitochondria. These alterations indicate possible tissue degeneration, demonstrating the cytotoxicity of thiamethoxam in the target and non target organs of newly emerged bees. Such results suggest cellular organelle impairment that can compromise cellular function of the midgut cells, Malpighian tubules cells and Kenyon cells, and, consequently, can compromise the longevity of the bees of the whole colony. (C) 2017 Elsevier Ltd. All rights reserved.