Magnetic-Field-Assisted Cellular Osteogenic Differentiation on Magnetic Zinc Ferrite Coatings via MEK/ERK Signaling Pathways.
Magnetic-Field-Assisted Cellular Osteogenic Differentiation on Magnetic Zinc Ferrite Coatings via MEK/ERK Signaling Pathways.
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DOI：
10.1021/acsbiomaterials.0c01087
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发表时间：
2020-11
影响因子：
5.8
通讯作者：
Bolin Tang;Xiaojun Shen;Guanchen Ye;Yaru Yang;Yang Jiang;Hongqin Xia;Xiaoyi Chen
中科院分区：
文献类型：
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作者：
Bolin Tang;Xiaojun Shen;Guanchen Ye;Yaru Yang;Yang Jiang;Hongqin Xia;Xiaoyi Chen
结合外部刺激和刺激响应生物材料可以调节细胞行为。本文设计了一种磁响应性铁酸锌（ZnFe 2 O 4）涂层，旨在研究静磁场作用下前成骨细胞的行为和成骨分化机制。通过在不同温度下退火制备了具有不同磁化强度（低、中、高磁化强度）的ZnFe 2 O 4涂层。细胞生物学实验表明，ZnFe_2 O_4涂层在SMF的辅助下均能促进MC 3 T3-E1细胞的早期增殖（3 d）和成骨分化。在不同ZnFe 2 O 4样品中，低、中磁化ZnFe 2 O 4样品在磁场作用下成骨相关基因（Runx 2、Col-I、OCN）的表达均高于高磁化ZnFe 2 O 4样品，而不同ZnFe 2 O 4样品上培养的细胞粘附和增殖差异无统计学意义。分子生物学检测表明，铁磁性ZnFe 2 O 4与SMF联合作用可显著提高α2β1整合素和p-ERK的表达水平。然而，添加抑制剂U 0126显著降低了p-ERK的表达水平，这表明α2β1整合素介导的MEK/ERK信号通路在ZnFe 2 O 4涂层的SMF辅助细胞成骨分化中起关键作用。这项工作提供了一个有吸引力的策略，以促进细胞成骨分化的远程控制的方式，这在骨组织修复和再生领域显示出巨大的潜力。
Combining an external stimulus and stimuli-responsive biomaterials can regulate cellular behaviors. In this paper, a magneto-responsive zinc ferrite (ZnFe2O4) coating was designed to gain insight into the preosteoblasts behaviors and osteogenic differentiation mechanism under a static magnetic field (SMF). ZnFe2O4 coatings with distinct magnetization (low, medium, and high magnetizations) were prepared by being annealed at different temperatures. Cellular biology experiments indicated that all ZnFe2O4 coatings with the assistance of SMF could promote the early proliferation (3 days) and osteogenic differentiation of MC3T3-E1 cells. Among different ZnFe2O4 samples, low and medium magnetization of ZnFe2O4 showed a higher osteogenesis-related gene expression (Runx2, Col-I, OCN) than that of high magnetization ZnFe2O4 under SMF, while cellular adhesion and proliferation cultured on different ZnFe2O4 samples presented insignificant differences. Molecular biology tests showed that the combination of ferromagnetic ZnFe2O4 and SMF could significantly improve the expression level of α2β1 integrin and p-ERK. However, the addition of the inhibitor U0126 sharply reduced the expression level of p-ERK, which indicated that α2β1 integrin-mediated MEK/ERK signaling pathways play a key role in SMF-assisted cellular osteogenic differentiation over ZnFe2O4 coatings. This work provides an attractive strategy to enhance cellular osteogenic differentiation in a remote-control way, which exhibited enormous potential in the field of bone tissue repair and regeneration.