Quantitative Label-Free Animal Sperm Imaging by Means of Digital Holographic Microscopy
Quantitative Label-Free Animal Sperm Imaging by Means of Digital Holographic Microscopy
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DOI：
10.1109/jstqe.2009.2036741
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发表时间：
2010-07-01
影响因子：
4.9
通讯作者：
Coppola, Giuseppe
中科院分区：
文献类型：
--
作者：
Di Caprio, Giuseppe;Gioffre, Mariano A.;Coppola, Giuseppe
利用数字全息显微镜（DHM）对牛精子细胞的形态图像进行了检索和分析。数字全息术是一种非接触式技术，能够在不改变其特征的情况下研究样品的形状，并首次用于检索精子细胞的定量形态信息。不同的精子已被分析，通过这种技术，使我们能够获得3-D图像与精确的地形细节和有价值的信息形态缺陷，提供与生物学的考虑。此外，通过利用微流控系统，数字全息技术已被用来分析未染色的精子在其自然的生理环境。通过DHM获得的精子形态学图像的详细信息预计将提供更好地了解各种生殖途径，这反过来又有助于改善不孕症管理。这可能构成了一种替代方法的基础，用于动物养殖业，旨在研究形态学特征和运动精子细胞的分选。
A digital holographic microscope (DHM) has been employed in the retrieval and analysis of morphological images of bovine's sperm cells. Digital holography is a noncontact technique capable of investigating the shape of the sample without altering its characteristics and has been used for the first time in retrieving quantitative morphological information of sperm cells. Different spermatozoa have been analyzed by means of this technique allowing us to obtain 3-D images with precise topographical details and valuable information about morphological defects, provided with biological considerations. Moreover, by making use of a microfluidic system, the digital holographic technique has been employed to analyze unstained spermatozoa in their natural physiological surroundings. Detailed information on morphological images of spermatozoa acquired by DHM is expected to provide a better understanding of various reproductive pathways, which, in turn, can help in improving infertility management. This could constitute the basis of an alternative method for the zoothecnic industry aimed at the investigation of morphological features and the sorting of the motile sperm cells.