Trypanosoma evansi: Paraflagellar rod protein 1 and 2 are similar but lack common B cell epitopes
Trypanosoma evansi: Paraflagellar rod protein 1 and 2 are similar but lack common B cell epitopes
复制标题
DOI：
10.1016/j.exppara.2008.08.007
复制
发表时间：
2008-12-01
影响因子：
2.1
通讯作者：
Suo, Xun
中科院分区：
文献类型：
--
作者：
Abdille, M. H.;Li, Shao Yong;Suo, Xun
为了鉴定具有疫苗潜力的非洲锥虫不变蛋白，我们研究了伊氏锥虫中PFR1蛋白的存在，并用免疫印迹和免疫沉淀法比较了其B细胞表位和伊氏锥虫的PFR2蛋白。根据布氏锥虫PFR1基因的开放阅读框设计了引物，通过RT-PCR扩增了伊氏锥虫的PFR1基因。克隆的伊氏锥虫PFR1基因与布氏锥虫和克氏锥虫的PFR1基因相似。表达的蛋白与伊氏锥虫PFR2蛋白的同源性为68.4%，与布氏锥虫、克氏锥虫、墨西哥利什曼原虫和大利什曼原虫的同源性分别为99.8%、87%、77.9%和77.5%。免疫印迹和免疫沉淀分析表明，在BALB/c小鼠中产生的针对PFR1和2蛋白的抗体分别识别PFR1和2蛋白，没有交叉反应。免疫凝集试验表明，抗PFR1、抗PFR2和抗天然PFR血清具有胰酶溶解特性。这些结果表明，PFR1和PFR2蛋白是天然PFR抗原的组成部分，不具有共同的B细胞表位。(C)2008 Elsevier Inc.保留所有权利。
In an attempt to identify invariant proteins with vaccine potential against African trypanosomes, we investigated the existence of PFR1 protein in Trypanosoma evansi and compared its B cell epitope with that of PFR2 protein of T. evansi using Western blotting and immuno-precipitation assays. The PFR1 gene of T. evansi was amplified by RT-PCR using primers designed based on the open reading frame of PFR1 gene of Trypanosoma brucei. The cloned PFR1 gene of T. evansi was similar to PFR1 genes of T. brucei and Trypanosoma cruzi. The expressed protein from the PFR1 gene was 68.4% homologous to the PFR2 protein of T. evansi, and showed 99.8%, 87%, 77.9% and 77.5% homologous to the PFR1 protein of T. brucei, T. cruzi, Leishmania mexicana and Leishmania major, respectively. Western blot and immuno-precipitation assays showed that antibodies raised against PFR1 and 2 proteins in BALB/c mice recognized the PFR1 and 2 proteins, respectively, with no cross-reactivity. Immuno-agglutination assay showed trypanolytic properties of the anti-PFR1, anti-PFR2 and anti-native PFR sera. These results suggest that PFR1 and PFR2 proteins are components of native PFR antigen and do not share common B cell epitopes. (C) 2008 Elsevier Inc. All rights reserved.