Membrane differentiations in freeze-fractured mammalian sperm.
Membrane differentiations in freeze-fractured mammalian sperm.
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DOI：
10.1083/jcb.63.2.641
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发表时间：
1974-11
期刊：
影响因子：
--
通讯作者：
Fawcett DW
中科院分区：
文献类型：
--
作者：
Friend DS;Fawcett DW
对豚鼠和大鼠精子进行了相关的薄切片和冷冻破裂研究。在切片上，顶体上的细胞膜上有一个刀豆蛋白a和钌红活性糖萼，其表现出与冻裂显示的膜平面内晶体结构域晶格相关的有序模式。顶体裂膜在某些区域也表现出较细的线性周期性。豚鼠顶体赤道段的膜上有斜脊栅栏，在大鼠身上没有观察到。顶体后区域的质膜中有丰富的膜嵌层颗粒，许多是随机分散的，另一些则呈直线排列。在后环的前方发现一个颗粒贫乏区。环后的冗余核膜褶皱上有许多紧密六边形排列的核孔。植入窝内的核包膜没有孔。当被切割时，它有一个无颗粒的中心区域，周围是一个大的、紧密排列的中空颗粒的广阔区域。豚鼠（而不是大鼠）的中间部分的膜包含6 - 8纳米的沿圆周定向的线性颗粒链。覆盖主片的膜显示出通常随机分布的颗粒，但除此之外，在膜内的外层致密纤维上纵向运行双排较大的（9纳米）颗粒。在薄片的相应位置，可以看到膜的局部增厚。这些观察结果为进一步研究精子膜这些区域特化的功能相关性奠定了基础。
A correlated thin-sectioning and freeze-fracturing study has been made of guinea pig and rat spermatozoa. In sections, the cell membrane over the acrosome has a concanavalin A and ruthenium red reactive glycocalyx which exhibits an ordered pattern related to the lattice of crystalline domains within the plane of the membrane revealed by freeze-fracturing. The cleaved acrosomal membrane also shows a finer linear periodicity in some areas. The membrane over the equatorial segment of the guinea pig acrosome is marked by a palisade of oblique ridges not observed in the rat. The plasmalemma of the postacrosomal region is rich in membrane intercalated particles, many randomly dispersed, others clustered in rectilinear arrays. A particle-poor zone is found just anterior to the posterior ring. The fold of redundant nuclear envelope posterior to the ring has many nuclear pores in close hexagonal array. The nuclear envelope lining the implantation fossa is devoid of pores. When cleaved it has a particle-free central area surrounded by a broad zone of large, closely packed, hollow particles. The membrane of the mid-piece in the guinea pig (but not the rat) contains linear strands of 6–8-nm particles oriented circumferentially. The membrane investing the principal piece exhibits the usual randomly distributed particles but in addition, a double row of larger (9 nm) particles runs longitudinally within the membrane over outer dense fiber 1. In the corresponding position in thin sections a local thickening of the membrane is discernible. These observations form a basis for further studies on the functional correlates of these regional specializations of the sperm membrane.