Decreased Bioenergetic Health Index in monocytes isolated from the pericardial fluid and blood of post-operative cardiac surgery patients.
Decreased Bioenergetic Health Index in monocytes isolated from the pericardial fluid and blood of post-operative cardiac surgery patients.
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DOI：
10.1042/bsr20150161
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发表时间：
2015-07-01
影响因子：
4
通讯作者：
Darley-Usmar VM
中科院分区：
文献类型：
--
作者：
Kramer PA;Chacko BK;George DJ;Zhi D;Wei CC;Dell'Italia LJ;Melby SJ;George JF;Darley-Usmar VM
转化生物能量学需要测量临床相关样本中的线粒体功能，并以可应用于个性化医疗的形式整合数据。在本研究中，我们显示的应用程序的测量的生物能量健康指数（BHI）的心脏手术患者。监测白细胞的生物能量学现已成为转化研究中检测血液或其他患者样本中线粒体功能障碍的重要方法。使用线粒体应激试验，其中包括顺序添加线粒体抑制剂粘附的白细胞，我们已经计算出一个单一的值，生物能量健康指数（BHI），它代表了从患者分离的细胞中的线粒体功能。在本报告中，我们评估了从接受心脏手术的患者的术后血液和术后心包液（PO-PCF）中分离的单核细胞的BHI。从患者的PO-PCF中分离的单核细胞的生物能量学分析显示，与从其血液或健康对照中分离的单核细胞相比，线粒体功能显著降低。此外，患者血液单核细胞在来自线粒体应激测试的个体能量参数中没有显示出显著差异，但是当整合到BHI评估中时，与健康对照单核细胞相比，BHI显著降低。这些数据支持BHI测量在将来自线粒体应激试验的个体参数整合为单个值中的效用。支持我们以前的发现，PO-PCF是促氧化剂，我们发现，大鼠心肌细胞暴露于PO-PCF引起线粒体膜电位的显着损失和活性氧（ROS）增加。这些研究结果支持的假设，生物能量健康的综合措施可能有预后和诊断价值的翻译生物能量学。
Translational bioenergetics requires the measurement of mitochondrial function in clinically relevant samples and the integration of the data in a form that can be applied to personalized medicine. In the present study, we show the application of the measurement of the Bioenergetic Health Index (BHI) to cardiac surgery patients. Monitoring the bioenergetics of leucocytes is now emerging as an important approach in translational research to detect mitochondrial dysfunction in blood or other patient samples. Using the mitochondrial stress test, which involves the sequential addition of mitochondrial inhibitors to adherent leucocytes, we have calculated a single value, the Bioenergetic Health Index (BHI), which represents the mitochondrial function in cells isolated from patients. In the present report, we assess the BHI of monocytes isolated from the post-operative blood and post-operative pericardial fluid (PO-PCF) from patients undergoing cardiac surgery. Analysis of the bioenergetics of monocytes isolated from patients’ PO-PCF revealed a profound decrease in mitochondrial function compared with monocytes isolated from their blood or from healthy controls. Further, patient blood monocytes showed no significant difference in the individual energetic parameters from the mitochondrial stress test but, when integrated into the BHI evaluation, there was a significant decrease in BHI compared with healthy control monocytes. These data support the utility of BHI measurements in integrating the individual parameters from the mitochondrial stress test into a single value. Supporting our previous finding that the PO-PCF is pro-oxidant, we found that exposure of rat cardiomyocytes to PO-PCF caused a significant loss of mitochondrial membrane potential and increased reactive oxygen species (ROS). These findings support the hypothesis that integrated measures of bioenergetic health could have prognostic and diagnostic value in translational bioenergetics.