Identification of membrane-bound carbonic anhydrase in white matter coated vesicles: the fate of carbonic anhydrase and other white matter coated vesicle proteins in triethyl tin-induced leukoencephalopathy.
Identification of membrane-bound carbonic anhydrase in white matter coated vesicles: the fate of carbonic anhydrase and other white matter coated vesicle proteins in triethyl tin-induced leukoencephalopathy.
复制标题
白质包被囊泡中膜结合碳酸酐酶的鉴定：碳酸酐酶和其他白质包被囊泡蛋白在三乙基锡诱导的白质脑病中的命运。
DOI：
10.1002/jnr.490350110
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发表时间：
1993
影响因子：
4.2
通讯作者：
Marks,N
中科院分区：
文献类型：
--
作者：
Sapirstein,VS;Durrie,R;Nolan,CE;Marks,N
我们扩展了对白质衍生包被囊泡 (WMCV) 含量的研究，表明它们富含膜结合碳酸酐酶。在髓鞘复合体中，膜结合碳酸酐酶集中在轴膜周围区域；然而，即使相对于髓磷脂膜区域，这种蛋白质在包被囊泡的双层中也富集了近七倍。这些数据表明一些囊泡源自该酶高度定位的位点。观察到的膜结合碳酸酐酶的富集是独特的，因为其他轴膜蛋白（例如 CNPase 和 plasmolipin）仅以等量存在于轴膜周围髓磷脂组分和 WMCV 中。根据已知的定位，WMCV 中 CNPase 的存在与 MAG 的缺乏表明这些囊泡源自结节旁区域。在 WMCV 中鉴定出与离子和液体运动相关的轴膜周髓鞘蛋白，例如碳酸酐酶、Na+、K+ATP 酶和假定的 K+通道蛋白血浆脂蛋白，促使我们检查这些囊泡在三乙基锡 (TET) 诱导的髓鞘水肿中的状态。从对照大鼠和 TET 治疗大鼠的全脑中分离出包被的囊泡和其他膜部分。使用全脑，以便我们可以比较 TET 对 WMCV 蛋白的影响与对富含灰质包被囊泡的蛋白质的影响。结果表明，TET 对致密髓磷脂或轴膜周围髓磷脂没有可检测到的影响，然而，蛋白质印迹分析表明，WMCV 蛋白，如碳酸酐酶、CNPase 和 plasmolipin，在全脑包被的囊泡部分中几乎不存在或大大减少。相比之下，灰质包被的囊泡蛋白（例如突触素、SV2）和一般囊泡蛋白、阳离子独立的甘露糖-6-磷酸受体则没有变化。这些结果表明，包被的囊泡可能是与白细胞病理学相关的膜变化的有用探针。 © 1993 Wiley-Liss, Inc.
We have extended our studies on the content of white matter derived coated vesicles (WMCVs) to show that they are enriched in membrane‐bound carbonic anhydrase. Within the myelin complex membrane‐bound carbonic anhydrase is concentrated in the periaxolemmal domain; however, this protein is enriched almost sevenfold in the bilayer of coated vesicles even relative to this myelin membrane region. These data suggest that some vesicles are derived from a site at which this enzyme is highly localized. The enrichment observed for membrane‐bound carbonic anhydrase is unique since other periaxolemmal proteins such as CNPase and plasmolipin are only present in equal amounts in periaxolemmal‐myelin fractions and WMCVs. Based on their known localization, the presence of CNPase coupled with the absence of MAG in WMCVs suggest that these vesicles are derived from the paranodal region. The identification in WMCVs of periaxolemmal‐myelin proteins associated with ion and fluid movement, such as carbonic anhydrase, Na+, K+ATPase, and the putative K+channel protein plasmolipin, prompted us to examine the status of these vesicles in triethyl tin (TET)‐induced myelin edema. Coated vesicles and other membrane fractions were isolated from whole brains of control and TET‐treated rats. Whole brains were used so we could compare the effects of TET on WMCV proteins with the effect on proteins enriched in gray matter coated vesicles. The results indicated that TET had no detectable effect on compact or periaxolemmal‐myelin, however, Western blot analysis showed that WMCV proteins, such as carbonic anhydrase, CNPase, and plasmolipin, were virtually absent or greatly diminished from the whole brain coated vesicle fraction. In contrast, gray matter coated vesicle proteins such as synaptophysin, SV2, and the general vesicle protein, cation‐independent mannose‐6‐phosphate receptor were unchanged. These results indicate that coated vesicles may be a useful probe for membrane changes associated with leukopathologies. © 1993 Wiley‐Liss, Inc.