Plasma Exosomal miRNA-139-3p is a Novel Biomarker of Colorectal Cancer
Plasma Exosomal miRNA-139-3p is a Novel Biomarker of Colorectal Cancer
复制标题
血浆外泌体 miRNA-139-3p 是结直肠癌的新型生物标志物
DOI：
10.7150/jca.45548
复制
发表时间：
2020-01-01
影响因子：
3.9
通讯作者：
Li, Dong
中科院分区：
文献类型：
--
作者：
Liu, Wanchao;Yang, Dianyu;Li, Dong
Objectives: This study investigated plasma exosomal miRNA-139-3p as a blood-based biomarker for the early diagnosis and metastasis monitoring of colorectal cancer (CRC).Patients and Methods: Exosome-rich fractions were isolated from the plasma of 80 CRC patients, and 23 controls using a kit method. We then used real-time polymerase chain reaction (RT-qPCR) to detect miR-139-3p levels in all subjects to evaluate expression levels and the predictive value of plasma exosomal miR-139-3p in CRC. We also collected clinicopathological data to explore correlations between abnormal miR-139-3p expression and clinicopathological parameters.Results: When compared with healthy controls, exosomal miR-139-3p expression levels in CRC patients were significantly down-regulated. Furthermore, these expression levels were lower in metastatic colorectal cancer (mCRC) and submucosal patients. Receiver operating characteristic (ROC) curve analysis showed that exosomal miR-139-3p levels were differentiated between CRC patients and healthy controls, as well as between non-metastatic and metastatic patients.Conclusion: Our findings show that decreased exosomal miR-139-3p expression levels in CRC patient plasma may act as a novel biomarker for the early diagnosis and metastasis monitoring in CRC.
