Eosinophil Cationic Protein Shows Survival Effect on H9c2 Cardiac Myoblast Cells with Enhanced Phosphorylation of ERK and Akt/GSK-3β under Oxidative Stress.
Eosinophil Cationic Protein Shows Survival Effect on H9c2 Cardiac Myoblast Cells with Enhanced Phosphorylation of ERK and Akt/GSK-3β under Oxidative Stress.
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DOI：
10.18926/amo/53521
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发表时间：
2015-03
影响因子：
0.5
通讯作者：
H. Ishii;Shigeshi Kamikawa;S. Hirohata;Akifumi Mizutani;K. Abe;M. Seno;T. Oohashi;Y. Ninomiya
中科院分区：
文献类型：
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作者：
H. Ishii;Shigeshi Kamikawa;S. Hirohata;Akifumi Mizutani;K. Abe;M. Seno;T. Oohashi;Y. Ninomiya
嗜酸性粒细胞阳离子蛋白（ECP）是嗜酸性粒细胞碱性颗粒中的一种阳离子蛋白。最近的研究报道，ECP表现出对各种类型的细胞，包括大鼠新生心肌细胞的新的活动。本实验观察体外反搏对大鼠心肌成肌细胞H9 c2的影响。我们的研究结果表明，ECP增强细胞的存活，部分通过促进ERK和Akt/GSK-3β信号通路。ECP能明显抑制H_2O_2对H9c_2细胞的毒性作用，并能抑制H_2O_2引起的细胞活性氧的产生、凋亡细胞数和caspase 3/7活性的增加。总之，ECP激活ERK和Akt/GSK-3β通路，导致对H9 c2细胞的抗氧化作用，从而减弱凋亡。
Eosinophil cationic protein (ECP) is well known as a cationic protein contained in the basic granules of activated eosinophils. Recent studies have reported that ECP exhibits novel activities on various types of cells, including rat neonatal cardiomyocytes. Here we evaluated the effects of ECP on rat cardiac myoblast H9c2 cells. Our results showed that ECP enhanced the survival of the cells, in part by promoting the ERK and Akt/GSK-3β signaling pathways. ECP attenuated the cytotoxic effects of H2O2 on H9c2 cells as well as the production of reactive oxygen species, the number of apoptotic cells and caspase 3/7 activity in the cells. In conclusion, ECP activated the ERK and Akt/GSK-3β pathways, resulting in anti-oxidative effects on H9c2 cells that attenuated apoptosis.