Probing Single Molecule Binding and Free Energy Profile with Plasmonic Imaging of Nanoparticles
Probing Single Molecule Binding and Free Energy Profile with Plasmonic Imaging of Nanoparticles
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利用纳米粒子的等离子体成像探测单分子结合和自由能分布
DOI：
10.1021/jacs.9b08405
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发表时间：
2019-10-09
影响因子：
15
通讯作者：
Tao, Nongjian
中科院分区：
文献类型：
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作者：
Wang, Hui;Tang, Zhuodong;Tao, Nongjian
测量分子之间的结合对于了解基本的生化过程、开发分子诊断和筛选药物至关重要。在这里，我们通过将纳米颗粒附着到分子结合对上来研究单分子水平上的分子结合。我们利用等离子体散射成像技术，以亚纳米精度跟踪单个纳米粒子的热涨落，结果表明，涨落是由分子结合对控制的，而不是由纳米粒子控制的。热涨落的分析提供了关于分子结合的独特信息，包括结合能分布、有效弹性常数以及单分子结合事件和多分子结合事件之间的切换。该方法为分子结合提供了新的见解，并使人们能够区分非特异性结合和特定结合，这一直是生物传感器中的一项困难任务。
Measuring binding between molecules is critical for understanding basic biochemical processes, developing molecular diagnosis, and screening drugs. Here we study molecular binding at the single molecule level by attaching nanoparticles to the molecular binding pairs. We track the thermal fluctuations of the individual nanoparticles with sub-nanometer precision using a plasmonic scattering imaging technique and show that the fluctuations are controlled by the molecular binding pairs rather than by the nanoparticles. Analysis of the thermal fluctuations provides unique information on molecular binding, including binding energy profile, effective spring constant, and switching between single and multiple molecular binding events. The method provides new insights into molecular binding and also allows one to differentiate nonspecific binding from specific binding, which has been a difficult task in biosensors.