Organohalide Respiration with Diclofenac by Dehalogenimonas
Organohalide Respiration with Diclofenac by Dehalogenimonas
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脱卤单胞菌与双氯芬酸进行有机卤化物呼吸
DOI：
10.1021/acs.est.1c08824
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发表时间：
2022
影响因子：
11.4
通讯作者：
Jun Yan
中科院分区：
文献类型：
--
作者：
Xiuying Li;Yi Yang;Jingjing Wang;Huijuan Jin;Yaozhi Zhang;Cui Yiru;Yufang Song;Jun Yan
双氯芬酸 (DCF) 是一种常见于水生生态系统中的药物活性污染物。 DCF 生物降解（特别是在缺氧条件下）所涉及的转化途径和微生物学仍然知之甚少。在这里，我们证明了在源自受污染河流沉积物的厌氧富集培养物中微生物介导的 DCF 还原脱氯。在 160 天的培养过程中，超过 90% 的初始 76.7 ± 3.6 μM DCF 在第 1 天最大脱氯率为 1.8 ± 0.3 μM。质谱分析证实，2-(2-((2-氯苯基)氨基)苯基)乙酸(2-CPA)和2-苯胺基苯乙酸(2-APA)分别作为单氯化和非氯化DCF转化产物形成。对微生物组成和桑格测序的调查揭示了一个新的脱卤单胞菌群体的富集和优势，该群体被命名为脱卤单胞菌属。 DCF 菌株，属于 DCF 脱氯群落。将 DCF 化学计量转化为 2-CPA (76.0 ± 2.1 μM) 和 2-APA (3.7 ± 0.8 μM) 后，菌株 DCF 细胞密度增加了 24.4 ± 4.4 倍，每释放 μmol 氯化物，生长产量为 9.0 ± 0.1 × 108 个细胞。我们的研究结果扩大了脱卤单胞菌属的代谢能力，并强调了有机卤化物呼吸细菌在自然减弱新出现的卤化污染物（例如 DCF）方面的相关作用。
Diclofenac (DCF) is a pharmaceutically active contaminant frequently found in aquatic ecosystems. The transformation pathways and microbiology involved in the biodegradation of DCF, particularly under anoxic conditions, remain poorly understood. Here, we demonstrated microbially mediated reductive dechlorination of DCF in anaerobic enrichment culture derived from contaminated river sediment. Over 90% of the initial 76.7 ± 3.6 μM DCF was dechlorinated at a maximum rate of 1.8 ± 0.3 μM day-1 during a 160 days' incubation. Mass spectrometric analysis confirmed that 2-(2-((2-chlorophenyl)amino)phenyl)acetic acid (2-CPA) and 2-anilinophenylacetic acid (2-APA) were formed as the monochlorinated and nonchlorinated DCF transformation products, respectively. A survey of microbial composition and Sanger sequencing revealed the enrichment and dominance of a new Dehalogenimonas population, designated as Dehalogenimonas sp. strain DCF, in the DCF-dechlorinating community. Following the stoichiometric conversion of DCF to 2-CPA (76.0 ± 2.1 μM) and 2-APA (3.7 ± 0.8 μM), strain DCF cell densities increased by 24.4 ± 4.4-fold with a growth yield of 9.0 ± 0.1 × 108 cells per μmol chloride released. Our findings expand the metabolic capability in the genus Dehalogenimonas and highlight the relevant roles of organohalide-respiring bacteria for the natural attenuation of halogenated contaminants of emerging concerns (e.g., DCF).