HSF3 is a major heat shock responsive factor during chicken embryonic development
HSF3 is a major heat shock responsive factor during chicken embryonic development
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DOI：
10.1046/j.1432-1327.1999.00762.x
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发表时间：
1999-10-01
期刊：
影响因子：
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通讯作者：
Nakai, A
中科院分区：
文献类型：
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作者：
Kawazoe, Y;Tanabe, M;Nakai, A
脊椎动物热休克基因的表达主要在转录水平上受4种热休克转录因子（HSFs - 1-4）的调控。禽细胞至少表达3种hsf （hsf 1-3）。即使是轻微的热休克也能迅速激活HSF1，而HSF3只有在严重的热休克时才会激活。相比之下，HSF2不被热应激激活，并被推测具有发育功能。在这里，我们研究了鸡发育过程中不同组织中这三种hsf水平的时空变化。我们发现HSF3在鸡发育早期和晚期的各种组织中几乎持续表达，HSF1在发育早期的大多数组织中表达同样高，随后以组织依赖的方式下降到不同的水平。因此，HSF3成为所有组织中主要的热反应因子。Northern印迹法测定的热休克反应程度并不总是与HSF1表达水平相关，提示除了HSF1外，HSF3也可能是介导鸡热休克基因表达的应激信号的主要因素。此外，HSF2以及HSF1和HSF3在胚胎早期的高水平和普遍表达表明这些因子参与了所有的发育过程。
The expression of heat shock genes in vertebrates is regulated mainly at the level of transcription by four heat shock transcription factors (HSFs 1-4). Avian cells express at least three HSFs (HSFs 1-3). HSF1 is rapidly activated by even mild heat shock, while HSF3 is activated only by severe heat shock. In contrast, HSF2 is not activated by heat stress and has been speculated to have developmental functions. Here, we examined the temporal and spatial profiles of changes in the levels of these three HSFs in various tissues during chicken development. We found that HSF3 was almost constantly expressed in various tissues during early to late chicken development, The expression of HSF1 was equally high in most tissues early in development and thereafter declined to different levels in a tissue-dependent manner. Thus, HSF3 became the dominant heat-responsive factor in all tissues examined. The magnitude of heat shock response determined by Northern blotting did not always correlate with the level of HSF1 expression, suggesting that not only HSF1 but also HSF3 may be a major factor mediating stress signals to heat shock gene expression in the chicken. In addition, the high-level and ubiquitous expression of HSF2 as well as HSF1 and HSF3 in early embryogenesis suggested the involvement of these factors in all developmental processes.