Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes
Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes
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DOI：
10.1016/j.dci.2017.03.007
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发表时间：
2017-08-01
影响因子：
2.9
通讯作者：
Miyazato, Mikiya
中科院分区：
文献类型：
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作者：
Kono, Tomoya;Ida, Takanori;Miyazato, Mikiya
本文研究了神经介肽U（Nmu）对红鳍东方鲀（Takifugu rubripes）的免疫调节作用。在河豚鱼中发现了nmu mRNA的三种剪接变体，分别由164个（Nmu 1）、139个（Nmu 2）和129个（Nmu 3）氨基酸残基组成，其生物活性C端区域与鱼类和其他脊椎动物的NMU具有高度的同源性。河豚nmu的基因组结构不同于斑马鱼和哺乳动物。但在系统发育分析中，除了neuromedin S外，河豚Nmu还与其他脊椎动物的NMU聚在一起。在各种组织中鉴定了mRNA的剪接变体。Nmu-21和Nmu-9是从河豚肠中纯化的内源性肽。合成的Nmu-21肽激活吞噬细胞，并提高外周血白细胞中细胞因子mRNA的表达。(C)2017爱思唯尔有限公司版权所有
In this study, immunoregulatory function of neuromedin U (Nmu) in the teleost fish Fugu (Takifugu rubripes) was characterized. Three splicing variants of nmu mRNA encoding preproNMUs consisting of 164 (Nmu1), 139 (Nmu2), and 129 (Nmu3) amino acid residues were found in Fugu.The biologically active C-terminal region of Fugu Nmu showed high homology among fish and other vertebrate NMUs. The genomic organization of Fugu nmu differed from those of zebrafish and mammals. However, in phylogenetic analysis, Fugu Nmu formed a cluster with NMUs of other vertebrates, in addition to neuromedin S. The splicing variants of mRNA were identified in various tissues. Nmu-21 and Nmu-9 were purified as endogenous peptides from Fugu intestine. The synthetic Nmu-21 peptide activated phagocytic cells, and elevated the expression of cytokine mRNA in peripheral blood leukocytes. (C) 2017 Elsevier Ltd. All rights reserved.