SIMPLE METHOD FOR COLLECTING INFECTIVE LARVAE OF LITOMOSOIDES-CARINII
SIMPLE METHOD FOR COLLECTING INFECTIVE LARVAE OF LITOMOSOIDES-CARINII
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DOI：
10.2307/3279423
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发表时间：
1976-01-01
影响因子：
1.3
通讯作者：
MCCALL, JW
中科院分区：
文献类型：
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作者：
MCCALL, JW
描述了一种方法，通过该方法大量感染L.卡氏杆菌可以相对容易地获得。在2个单独的实验中，通过将5或7只蒙古沙鼠（长爪沙鼠）暴露于感染的热带鼠螨（Ornithonyssus bacoti）6或7 h的毛皮浸泡，回收了> 5000只感染性幼虫。螨感染7-8或9-10天前，暴露于沙鼠与专利感染。在感染的幼虫总数中，94%在2 1/2小时内恢复并迁移出毛皮。皮下接种的沙鼠以这种方式收集的感染性幼虫在尸检时表现出高的平均回收率，即，79和60在接种后5和29天。蠕虫回收率范围较窄。
A method is described whereby substantial quantities of infective larvae of L. carinii can be obtained with relative ease. In each of 2 separate experiments, > 5000 infective larvae were recovered by soaking the pelts of 5 or 7 Mongolian jirds (Meriones unguiculatus) exposed for 6 or 7 h to infected tropical rat mites (Ornithonyssus bacoti). The mites were infected 7-8 or 9-10 days earlier by exposure to jirds with patent infections. Of the total number of infective larvae, 94% recovered and migrated out of the pelts within 2 1/2 h. Jirds inoculated s.c. with infective larvae collected in this manner exhibited high mean percent recoveries at necropsy, i.e., 79 and 60 at 5 and 29 days postinoculation, respectively. The ranges in worm recoveries were narrow.