Comparison of subcutaneous and intraperitoneal injection of d-luciferin for in vivo bioluminescence imaging
Comparison of subcutaneous and intraperitoneal injection of d-luciferin for in vivo bioluminescence imaging
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DOI：
10.1007/s00259-008-1022-8
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发表时间：
2009-05-01
影响因子：
9.1
通讯作者：
Ohtomo, Kuni
中科院分区：
文献类型：
--
作者：
Inoue, Yusuke;Kiryu, Shigeru;Ohtomo, Kuni
我们比较了皮下（SC）注射和腹腔（IP）注射d-荧光素用于体内生物发光成像（BLI），以确定SC注射的效用。携带稳定表达萤火虫荧光素酶的SC肿瘤的小鼠通过SC和IP注射d-荧光素进行体内BLI。BLI研究每隔3小时重复一次，使用给定的注射途径来评估可重复性，使用不同的注射途径来评估相关性。在患有SC和IP肿瘤的小鼠中，分别通过静脉注射（IV）、SC和IP注射d-荧光素进行BLI。血液恶性肿瘤模型小鼠采用SC和IP注射进行BLI。在SC肿瘤中，SC注射比IP注射的峰值时间稍短，峰值信号更大。两种注射方式测定峰信号的重复性具有可比性，且具有良好的相关性。在患有SC和IP肿瘤的小鼠中，IP肿瘤的信号相对于SC肿瘤的信号，使用IP注射比使用IV或SC注射要大得多。在血液学恶性肿瘤模型中，来自脾脏的信号相对于来自骨髓的信号，IP注射比SC注射更大。除了罕见的注射失败外，注射d-荧光素会导致对IP组织信号的高估。对于BLI， SC注入被证明是IP注入的一种方便的替代方法。
We compared subcutaneous (SC) injection and intraperitoneal (IP) injection of d-luciferin for in vivo bioluminescence imaging (BLI) to determine the utility of SC injection.Mice bearing SC tumours stably expressing firefly luciferase underwent in vivo BLI using SC and IP injection of d-luciferin. BLI studies were repeated at an interval of 3 h using a given injection route to assess repeatability and using different injection routes to assess correlation. In mice bearing both SC and IP tumours, BLI was performed successively using intravenous (IV), SC, and IP injection of d-luciferin. Haematological malignancy model mice underwent BLI using SC and IP injection.In SC tumours, the peak time was slightly shorter and the peak signal was greater using SC injection than using IP injection. The repeatability of determining peak signals was comparable between the two injection routes, and a good correlation was observed between them. In mice bearing both SC and IP tumours, signals from IP tumours relative to those from SC tumours were much greater using IP injection than using IV or SC injection. In the haematological malignancy model, signals from the spleen relative to those from the bone marrow were greater using IP injection than using SC injection.In addition to rare injection failure, the IP injection of d-luciferin led to the overestimation of signals from IP tissues. For BLI, SC injection was shown to be a convenient alternative to IP injection.