THE DROSOPHILA-MELANOGASTER GYPSY TRANSPOSABLE ELEMENT ENCODES PUTATIVE GENE-PRODUCTS HOMOLOGOUS TO RETROVIRAL PROTEINS
THE DROSOPHILA-MELANOGASTER GYPSY TRANSPOSABLE ELEMENT ENCODES PUTATIVE GENE-PRODUCTS HOMOLOGOUS TO RETROVIRAL PROTEINS
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DOI：
10.1128/mcb.6.4.1129
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发表时间：
1986-04-01
影响因子：
5.3
通讯作者：
CORCES, VG
中科院分区：
文献类型：
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作者：
MARLOR, RL;PARKHURST, SM;CORCES, VG
我们确定了完整的核苷酸序列的吉普赛元素目前在分叉的基因座果蝇的f1等位基因。gypsy元件与脊椎动物逆转录病毒的同源性高于D.黑腹酵母的Ty元件或酿酒酵母的Ty元件，无论是在整体组织还是在DNA序列水平。这个转座因子长7，469个碱基对，编码三种推定的蛋白质产物。长末端重复序列长482个核苷酸，含有转录起始和终止信号;与逆转录病毒的多尿区和tRNA引物结合位点同源的序列位于长末端重复序列附近。元件的中心区域包含三个不同的开放阅读框架。第二个编码一个推定的蛋白质，该蛋白质与逆转录病毒蛋白质具有广泛的氨基酸同源性，包括gag特异性蛋白酶、逆转录酶和DNA内切酶。
We determined the complete nucleotide sequence of the gypsy element present at the forked locus of Drosophila melanogaster in the f1 allele. The gypsy element shares more homology with vertebrate retroviruses than with the copia element of D. melanogaster or the Ty element of Saccharomyces cerevisiae, both in overall organization and at the DNA sequence level. This transposable element is 7,469 base pairs long and encodes three putative protein products. The long terminal repeats are 482 nucleotides long and contain transcription initiation and termination signals; sequences homologous to the polyurine tract and tRNA primer binding site of retroviruses are located adjacent to the long terminal repeats. The central region of the element contains three different open reading frames. The second one encodes a putative protein which shows extensive amino acid homology to retroviral proteins, including gag-specific protease, reverse transcriptase, and DNA endonuclease.