A novel alginate from Sargassum seaweed promotes diabetic wound healing by regulating oxidative stress and angiogenesis.
A novel alginate from Sargassum seaweed promotes diabetic wound healing by regulating oxidative stress and angiogenesis.
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DOI：
10.1016/j.carbpol.2022.119437
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发表时间：
2022-04
影响因子：
11.2
通讯作者：
Xuxiu Lu;Ling Qin;Meng Guo;Jiajia Geng;Songtao Dong;Kai Wang;Hui Xu;Chang-feng Qu;J. Miao;Mingju Liu
中科院分区：
文献类型：
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作者：
Xuxiu Lu;Ling Qin;Meng Guo;Jiajia Geng;Songtao Dong;Kai Wang;Hui Xu;Chang-feng Qu;J. Miao;Mingju Liu
糖尿病皮肤溃疡是糖尿病最严重的并发症之一，目前的治疗方法尚不完善。调节氧化应激、炎症和血管生成的药剂对于减轻糖尿病皮肤溃疡是相当有希望的。本研究制备了一种新的海藻多糖（SARP）。SARP是具有45.4kDa的Mw的藻酸盐，由76.56%甘露糖醛酸、18.89%古罗糖醛酸和4.55%葡萄糖醛酸组成。SARP可通过激活核因子红细胞2相关因子2（Nrf 2）减轻氧化应激诱导的细胞损伤。SARP还促进HUVECs的迁移和管腔形成，这与血管内皮生长因子（VEGF）表达增加有关。在糖尿病伤口模型中，SARP（iv，200 mg/kg）给药增加血管生成，减轻氧化应激，改善糖尿病相关畸变，从而加速糖尿病伤口愈合。这些发现确定SARP有潜力被开发为糖尿病皮肤溃疡的候选药物。
Diabetic skin ulcer is one of the most severe complications in diabetes, however, current therapeutic approaches are not effective enough. Agents modulating oxidative stress, inflammation, and angiogenesis are quite promising for alleviation of diabetic skin ulcers. In this study, a novel Sargassumkjellmanianum-derived polysaccharide (SARP) was prepared. SARP was an alginate with Mw of 45.4 kDa, consisting of 76.56% mannuronic acid, 18.89% guluronic acid, and 4.55% glucuronic acid. SARP could attenuate oxidative stress-induced cell damage via activating nuclear factor erythroid 2-related factor 2 (Nrf2). SARP also promoted the migration and tube formation of HUVECs, which was related to the increased vascular endothelial growth factor (VEGF) expression. In diabetic wound model, SARP (iv, 200 mg/kg) administration increased angiogenesis, alleviated oxidative stress, ameliorated diabetes-related aberrations, and thereby accelerated diabetic wound healing. These findings identified SARP had potential to be developed as a drug candidate for diabetic skin ulcers.