Effect of nicotine on the expression of Thl/Th2 cytokines and transcription factor T-bet/GATA-3 incultured CD4＋ T cell of rat sensitized by ovalbumin
Effect of nicotine on the expression of Thl/Th2 cytokines and transcription factor T-bet/GATA-3 incultured CD4＋ T cell of rat sensitized by ovalbumin
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尼古丁对卵清蛋白致敏大鼠CD4＋T细胞Thl/Th2细胞因子及转录因子T-bet/GATA-3表达的影响
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
许建英
中科院分区：
文献类型：
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作者：
蒋毅;杜永成;许建英
Objective To investigate the effects of nicotine on the expression of Thl/Th2 cytokines and transcription factor T-bet/GATA-3 in cultured CIM＋ T of rat sensitized by ovalbumin. Methods Two weeks after immunization by ovalbumin, splenic CIM＋ T cells of Wistar rat were purified using CIMT cell enrichment kit. Purified CIMT cells of rat were cultured and divided into 4 groups： a control group, 1 pLg/ml nicotine stimulated group, 10 μg/ml nicotine stimulated group, 100μg/ml nicotine stimulated group. These cells, in their groups, were stimulated with or without nicotine and were all challenged simultaneously with OVA. Supernatants and cell pellets were harvested after being stimulated for 24 h. The concentration of IFN-y and IL-4 in supernatants were measured by enzyme-linked immunosorbent assay （ELISA）. Real-time PCR was used to determine the mRNA expression of T-bet and GATA-3 in CD4T cells. Results （ 1 ） IFN-y production was significantly decreased in all nicotine treated groups [ （ 113.78±6.06） ng/L, （70.31±7.26） ng/L, （20.00±2.14） ng/L] compared with the control group[ （ 142.30±5.89） ng/L] , and the level of IL-4 was significantly increased in all nicotine treated groups[ （69.49±3.91 ） ng/L, （93.63±4.56 ） ng/L, （ 50.97±3.07 ） ng/L ] compared with the control group [ （ 36.91 ±3.24） ng/L]. （2） Expression of T-bet mRNA in all nicotine treated groups（0.73±0.03, 0. 57± 0. 04 , 0. 31±0. 00 ） was lower than that in the control group（0.98±0.09） , but expression of GATA-3 mRNA in all nicotine treated groups（4.31±0.26, 5.16±0.23, 1.56±0.14） was significantly higher than that in the control group （1.00±0.07）. Conclusion Nicotine may play a key role in the development of Th2-type allergic inflammation in asthma by promoting over-expression of GATA-3 mRNA and downregulating the expression of T-bet mRNA.
Key words:
Nicotine ; Asthma ; T helper cell; Transcription factor
