Glycosidases in Carbohydrate Synthesis: When Organic Chemistry Falls Short
Glycosidases in Carbohydrate Synthesis: When Organic Chemistry Falls Short
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DOI：
10.2533/chimia.2011.65
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发表时间：
2011-01-01
期刊：
影响因子：
1.2
通讯作者：
Kren, Vladimir
中科院分区：
文献类型：
--
作者：
Bojarova, Pavla;Kren, Vladimir
由于稳定性、良好的可用性、立体选择性和广泛的底物特异性，糖苷酶催化的寡糖合成代表了一种复杂碳水化合物结构的优雅方法。提出了两种糖苷酶催化方法：i) 使用分子中携带各种功能部分的结构修饰底物，以及 ii) 设计没有水解活性的突变糖苷酶。糖苷酶催化合成的产品可应用于免疫学、阿尔茨海默病或帕金森病的治疗以及新糖蛋白的合成等一系列领域。
Thanks to the stability, good availability, stereoselectivity and broad substrate specificity, oligosaccharide synthesis catalyzed by glycosidases represents an elegant way to complex carbohydrate structures. Two approaches to glycosidase catalysis are presented: i) the use of structurally modified substrates that carry various functional moieties in the molecule, and ii) the design of mutant glycosidases void of hydrolytic activity. Products of glycosidase-catalyzed synthesis are applicable in a range of areas such as immunology, therapy of Alzheimer's or Parkinson's diseases and the synthesis of neoglycoproteins.