LOCATION AND NUCLEOTIDE-SEQUENCE OF THE GENE FOR THE PROTON-TRANSLOCATING SUBUNIT OF WHEAT CHLOROPLAST ATP SYNTHASE
LOCATION AND NUCLEOTIDE-SEQUENCE OF THE GENE FOR THE PROTON-TRANSLOCATING SUBUNIT OF WHEAT CHLOROPLAST ATP SYNTHASE
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DOI：
10.1073/pnas.79.22.6903
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
GRAY, JC
中科院分区：
文献类型：
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作者：
HOWE, CJ;AUFFRET, AD;GRAY, JC
小麦叶绿体ATP合酶的质子易位亚基是由一个叶绿体基因编码的，该基因已被精确定位并确定了其核苷酸序列。通过对该蛋白NH2端前40个氨基酸序列的测定，部分证实了所预测的81个氨基酸序列与已知菠菜蛋白的同源性为100%，与细菌和线粒体蛋白的同源性不超过35%。该基因没有偏离通用遗传密码，也没有分裂。潜在的核糖体结合位点位于起始密码子上游12个核苷酸处，但与原核启动子和转录终止子同源的序列并不明显。
The proton-translocating subunit of wheat chloroplast ATP synthase is encoded by a chloroplast gene that has been accurately mapped and whose nucleotide sequence has been determined. The predicted sequence of 81 amino acids has been confirmed in part by determination of the sequence of the 1st 40 amino acids from the NH2 terminus of the protein, and its shows 100% homology with the known amino acid sequence of the spinach protein but no more than 35% homology with the amino acid sequences of bacterial and mitochondrial proteins. The gene shows no deviation from the universal genetic code and is not split. A potential ribosome binding site is located 12 nucleotides upstream from the initiation codon, but sequences homologous to prokaryotic promotors and transcription terminators are not apparent.