MOLECULAR RELATIONSHIP OF CHROMOSOMAL GENES ENCODING BIPHENYL POLYCHLORINATED BIPHENYL CATABOLISM - SOME SOIL BACTERIA POSSESS A HIGHLY CONSERVED BPH OPERON
MOLECULAR RELATIONSHIP OF CHROMOSOMAL GENES ENCODING BIPHENYL POLYCHLORINATED BIPHENYL CATABOLISM - SOME SOIL BACTERIA POSSESS A HIGHLY CONSERVED BPH OPERON
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DOI：
10.1128/jb.171.10.5467-5472.1989
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发表时间：
1989-10-01
影响因子：
3.2
通讯作者：
TOMIZUKA, N
中科院分区：
文献类型：
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作者：
FURUKAWA, K;HAYASE, N;TOMIZUKA, N
我们研究的编码联苯/多氯联苯(PCB)降解的所有基因都是染色体，不同于许多其他编码降解的基因，这些基因是由质粒携带的。研究了降解联苯/多氯联苯的假单胞菌、嗜铬杆菌、产碱杆菌、莫拉氏菌和节杆菌中联苯/多氯联苯分解代谢编码基因的分子关系。在15株供试菌株中，5株假单胞菌和1株产碱假单胞菌在Xhol7.2kb碱基上具有与假单胞菌KF707相同的bphABC基因簇。更重要的是，这些包含bphABC基因的XhoI 7.2kb片段的限制性内切酶图谱即使不完全相同，也是非常相似的，尽管侧翼染色体区域不同。另外3株与KF707的bphABC基因也具有同源性，另外5株与KF707的bphABC基因同源性较弱或无显著差异。不同菌株的2，3-二羟基联苯双加氧酶的免疫交叉反应性与DNA同源性很好地吻合。另一方面，另一株降解多氯联苯的假单胞菌Q1的bphc基因与所测试的其他15株联苯/多氯联苯降解菌中的任何一株缺乏遗传和免疫同源性。不同菌株之间存在几乎相同的染色体基因，这可能表明包含bphABC基因的片段具有将基因从一个菌株转移到另一个菌株的机制。
All the genes we examined that encoded biphenyl/polychlorinated biphenyl (PCB) degradation were chromosomal, unlike many other degradation-encoding genes, which are plasmid borne. The molecular relationship of genes coding for biphenyl/PCB catabolism in various biphenyl/PCB-degrading Pseudomonas, Achromobacter, Alcaligenes, Moraxella, and Arthrobacter strains was investigated. Among 15 strains tested, 5 Pseudomonas strains and one Alcaligenes strain possessed the bphABC gene cluster on the Xhol 7.2-kilobase fragment corresponding to that of Pseudomonas pseudoalcaligenes KF707. More importantly, the restriction profiles of these XhoI 7.2-kilobase fragments containing bphABC genes were very similar, if not identical, despite the dissimilarity of the flanking chromosomal regions. Three other strains also possessed bphABC genes homologous with those of KF707, and five other strains showed weak or no significant genetic homology with bphABC of KF707. The immunological cross-reactivity of 2,3-dihydroxybiphenyl dioxygenases from various strains corresponded well to the DNA homology. On the other hand, the bphC gene of another PCB-degrading strain, Pseudomonas paucimobilis Q1, lacked genetic as well as immunological homology with any of the other 15 biphenyl/PCB degraders tested. The existence of the nearly identical chromosomal genes among various strains may suggest that a segment containing the bphABC genes has a mechanism for transferring the gene from one strain to another.