Self-normalization of highly transformed 3T3 cells through maximized contact interaction.
Self-normalization of highly transformed 3T3 cells through maximized contact interaction.
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通过最大化接触相互作用实现高度转化的 3T3 细胞的自我正常化。
DOI：
10.1073/pnas.79.6.1903
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发表时间：
1982
影响因子：
11.1
通讯作者：
Chu,BM
中科院分区：
文献类型：
--
作者：
Rubin,H;Chu,BM
未转化、中度转化和高度转化的BALB/c 3T3细胞在大容量培养基中维持在小盖上，分别比在常规培养中增加2倍、3倍和4倍的种群密度。剥夺Mg2+会导致覆盖层上高度转化的细胞呈现出未转化细胞的外观，降低其增殖速度，并以比生理Mg2+中的相同细胞低得多的饱和密度停止进一步生长。后一种细胞达到10(6)/cm2的饱和密度，其DNA合成速率随着拥挤程度的增加而逐渐降低。在饱和密度下，生理Mg2+细胞呈现出与正常成纤维细胞相似的外观和排列。他们对血清有很高的要求，以启动DNA合成。当以低密度转移时，它们在塑料表面变平，并保持未转化细胞的外观约1天。DNA合成和增殖在转移的细胞中开始延迟，这是受新鲜培养基或转移刺激的静止未转化细胞的特征。来自拥挤盖层的细胞悬浮在琼脂中的集落形成效率约为来自未拥挤盖层的细胞的1/10。它们的Mg2+含量也明显较低。低密度转移2 ~ 3天后，拥挤的细胞恢复了转化后的形态和生长行为。我们得出结论，非常高的拥挤程度导致高度转化的细胞恢复到非转化细胞的表型。其他处理如剥夺Mg2+或无机正磷酸盐也能达到类似的效果。似乎代谢率和增殖率的平衡降低可以恢复转化细胞的正常表型，这意味着它们只是在数量上与非转化细胞不同。讨论了Mg2+在动物细胞增殖和转化中的调节作用。
Nontransformed and moderately and highly transformed BALB/c 3T3 cells maintained on small coverslips in a large volume of medium multiplied to 2, 3, and 4 times higher population density, respectively, than they did in conventional cultures. Deprivation of Mg2+ caused highly transformed cells on coverslips to assume the appearance of nontransformed cells, decrease their rate of multiplication, and stop further growth at a much lower saturation density than the same cells in physiological Mg2+. The latter cells reached a saturation density of 10(6)/cm2 and their rate of DNA synthesis decreased progressively with increased crowding. At saturation density, cells in physiological Mg2+ took on an appearance and arrangement similar to normal fibroblasts. They developed a high requirement for serum to initiate DNA synthesis. When transferred at low density, they flattened out on a plastic surface and maintained the appearance of nontransformed cells for approximately 1 day. Onset of DNA synthesis and multiplication in the transferred cells was delayed for periods characteristic of quiescent nontransformed cells stimulated by fresh medium or transfer. Cells from crowded coverslips were approximately 1/10th as efficient at colony formation when suspended in agar as cells from uncrowded coverslips. They also had a significantly lower Mg2+ content. The crowded cells returned to their transformed morphological and growth behavior 2 to 3 days after transfer at low density. We conclude that a very high degree of crowding causes highly transformed cells to revert to the phenotype of nontransformed cells. Other treatments such as deprivation of Mg2+ or inorganic orthophosphate can achieve similar results. It appears that a balanced reduction in rates of metabolism and multiplication can restore the normal phenotype to transformed cells, implying that they differ only quantitatively from nontransformed cells. The putative role of Mg2+ in the regulation of multiplication and in transformation of animal cells is discussed.