Bacteria Associated with Hazelnut (Corylus avellana L.) Decline Are of Two Groups: Pseudomonas avellanae and Strains Resembling P. syringae pv. syringae
Bacteria Associated with Hazelnut (Corylus avellana L.) Decline Are of Two Groups: Pseudomonas avellanae and Strains Resembling P. syringae pv. syringae
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与榛子（Corylus avellana L.）衰退相关的细菌有两类： avellanae 假单胞菌和类似 P. syringae pv. 的菌株。
DOI：
10.1128/aem.68.2.476-484.2002
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发表时间：
2002
影响因子：
4.4
通讯作者：
P. Di Prospero
中科院分区：
文献类型：
--
作者：
M. Scortichini;U. Marchesi;M. Rossi;P. Di Prospero
摘要通过对118株在希腊北方和意大利不同地区已发生多年的与榛子衰退相关的荧光假单胞菌进行重复PCR分析，包括肠杆菌重复基因间共有序列、盒元件、重复基因外回文引物组、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳全细胞蛋白质提取物的SDS-PAGE分析、碳化合物利用分析和确定CCLB基因存在的分析。一个子集的53株也进行了表征扩增16 S核糖体DNA限制性分析（ARPGEX），通过使用9种限制性内切酶。采用连续剂量法对40株代表菌株进行毒力评价。同时也验证了菌株的致病特异性。用HinfI进行的ARFI显示了两个主要的菌株群，A群和B群，它们表现出57%的相似性。使用的其他八种限制性内切酶不能分离菌株。此外，在重复PCR和蛋白质提取物的SDS-PAGE后，使用算术平均值通过未加权对组方法进行的聚类分析也显示了相同的两个组。此外，一些碳化合物的差别利用使得区分这些组成为可能。毒力评估清楚地表明，A组菌株是非常强的毒力，而B组菌株被证明是轻度毒力的榛子。A组包括在希腊北方和意大利中部分离的菌株（即，维泰博省）;这些菌株不具有p53 B基因，在病原学上局限于欧洲榛，属于欧洲榛假单胞菌。组B包括从在皮埃蒙特、坎帕尼亚、西西里、西西里和撒丁岛栽培的榛子中获得的其它菌株。它们代表了与假单胞菌pv.你好
ABSTRACT A total of 118 fluorescent pseudomonads associated with hazelnut decline, which has been occurring for many years in different areas of northern Greece and Italy, were assessed by performing a repetitive PCR analysis with enterobacterial repetitive intergenic consensus, box element, and repetive extragenic palindromic primer sets, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell protein extracts, a carbon compound utilization analysis, and an analysis to determine the presence of the syrB gene. A subset of 53 strains was also characterized by amplified 16S ribosomal DNA restriction analysis (ARDRA) by using nine restriction endonucleases. The virulence of 40 representative strains was assessed by using serial doses. The pathogenic specificities of the strains were also verified. ARDRA carried out with HinfI revealed two main groups of strains, groups A and B, which exhibited a level of similarity of 57%. The other eight restriction endonucleases used did not separate the strains. In addition, a cluster analysis performed by the unweighted pair group method using arithmetic averages after repetitive PCR and SDS-PAGE of protein extracts also revealed the same two groups. Furthermore, the differential utilization of some carbon compounds made it possible to differentiate the groups. Virulence assessment clearly indicated that the group A strains are very virulent, whereas the group B strains proved to be mildly virulent for hazelnut. Group A included the strains isolated in northern Greece and central Italy (i.e., the province of Viterbo); these strains do not have the syrB gene, are pathogenically restricted to Corylus avellana, and belong to Pseudomonas avellanae. Group B includes the other strains obtained from hazelnut cultivated in Piedmont, Campania, Latium, Sicily, and Sardinia. They represent a distinct taxon closely related to Pseudomonas syringae pv. syringae.