Congenicity and genetic polymorphism in cloned lines derived from a single isolate of a rodent malaria parasite
Congenicity and genetic polymorphism in cloned lines derived from a single isolate of a rodent malaria parasite
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DOI：
10.1016/j.molbiopara.2007.10.011
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发表时间：
2008-02-01
影响因子：
1.5
通讯作者：
Carter, Richard
中科院分区：
文献类型：
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作者：
Pattaradilokrat, Sittiporn;Cheesman, Sandra J.;Carter, Richard
许多最常研究的啮齿动物疟原虫约氏疟原虫品系均源自命名为 17X 的单一寄生虫分离株。然而，在这些品系中，有一些寄生虫表现出基因型和表型（例如生长速度）的变化。我们在此描述了生长速率不同的 17X 克隆系之间使用特定基因的核苷酸序列和全基因组扩增片段长度多态性 (AFLP) 模式进行的比较遗传分析的结果。我们的研究结果表明 17X 的原始种群包含两种不相关的基因型。基因型 1 代表具有缓慢生长表型（例如 17X (NIMR)）和快速生长表型（例如 17XYM）的寄生虫。在该基因型中，还存在基因组差异，表现为少量 AFLP 带，可区分快速生长和缓慢生长的品系。另一种基因型，基因型 2，仅由具有缓慢生长表型的寄生虫代表（例如 17XA）。 (c) 2007 Elsevier B.V. 保留所有权利。
Many of the most commonly studied lines of the rodent malaria parasite Plasmodium yoelii yoelli originated from a single parasite isolate designated 17X. Amongst these lines, however, are parasites that exhibit variation in genotype and phenotype (e.g. growth rate). We describe here the results of a comparative genetic analysis between cloned lines of 17X that differ in growth rate, using nucleotide sequences of specific genes and patterns of genome-wide amplified fragment length polymorphism (AFLP). Our findings indicate that the original stock of 17X comprises two unrelated genotypes. Genotype-1 is represented by parasites with a slow growth phenotype (e.g. 17X (NIMR)) and a fast growth phenotype (e.g. 17XYM). Within this genotype, there are also genomic differences manifest as a small number of AFLP bands that differentiate the fast- and slow-growing lines from each other. The other genotype, genotype-2, is represented only by parasites with a slow growth phenotype (e.g. 17XA). (c) 2007 Elsevier B.V. All rights reserved.