Light response, oxidative stress management and nucleic acid stability in closely related Linderniaceae species differing in desiccation tolerance
Light response, oxidative stress management and nucleic acid stability in closely related Linderniaceae species differing in desiccation tolerance
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DOI：
10.1007/s00425-012-1628-8
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发表时间：
2012-08-01
期刊：
影响因子：
4.3
通讯作者：
Bartels, Dorothea
中科院分区：
文献类型：
--
作者：
Dinakar, Challabathula;Bartels, Dorothea
在本研究中，比较了三种密切相关的林德尼亚科物种对干燥敏感性不同的光和氧化应激防御反应。 Lindernia brevidens 是一种耐干燥植物，与 Craterostigma plantagineum（耐干燥）和 Lindernia subracemosa（干燥敏感）相比，在长日照条件下，叶子表现出强烈的紫色色素沉着。叶子中强烈的色素沉着并不影响耐干燥行为，但似乎与氧化应激保护有关。短日照植物的绿叶和长日照植物的紫叶为比较基本光合参数提供了合适的材料。紫色叶子中非光化学猝灭的增加似乎可以防止光抑制。用甲基紫精处理降低了长日照和短日照植物的光化学活性，但积累花青素的长日照植物比短日照植物保持了更高的非光化学猝灭。在短日照和长日照生长的植物中，干燥诱导的蛋白质和参与碳水化合物代谢的蛋白质的表达没有观察到差异，而编码叶绿体局部应激蛋白的转录本和编码抗氧化酶的转录本的表达存在差异。在车前草中，编码抗氧化酶的基因的表达在干燥过程中要么是组成型的，要么是上调的，而在 L. subracemosa 中，表达是下调的。 RNA 表达分析表明，L. subracemosa 在干燥过程中 mRNA 发生降解，但在耐干燥物种中则不然。这些结果表明更好的氧化应激管理和 mRNA 稳定性与干燥耐受性相关。
In the present study, three closely related Linderniaceae species which differ in their sensitivity to desiccation are compared in response to light and oxidative stress defence. Lindernia brevidens, a desiccation-tolerant plant, displayed intense purple pigmentation in leaves under long-day conditions in contrast to Craterostigma plantagineum (desiccation tolerant) and Lindernia subracemosa (desiccation sensitive). The intense pigmentation in leaves does not affect the desiccation tolerance behaviour but seems to be related to oxidative stress protection. Green leaves of short-day and purple leaves of long-day plants provided suitable material for comparing basic photosynthetic parameters. An increase in non-photochemical quenching in purple leaves appears to prevent photoinhibition. Treatment with methyl viologen decreased the photochemical activities in both long-day and short-day plants but long-day plants which accumulate anthocyanins maintained a higher non-photochemical quenching than short-day plants. No differences were seen in the expression of desiccation-induced proteins and proteins involved in carbohydrate metabolism in short-day and long-day grown plants, whereas differences were observed in the expression of transcripts encoding chloroplast-localised stress proteins and transcripts encoding antioxidant enzymes. While the expression of genes encoding antioxidant enzymes were either constitutive or up-regulated during desiccation in C. plantagineum, the expression was down-regulated in L. subracemosa. RNA expression analysis indicated degradation of mRNA during desiccation in L. subracemosa but not in desiccation tolerant species. These results indicate that a better oxidative stress management and mRNA stability are correlated with desiccation tolerance.