DNA hypomethylation circuit of mouse rDNA repeats in the germ cell lineage.
DNA hypomethylation circuit of mouse rDNA repeats in the germ cell lineage.
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DOI：
10.1016/j.bbrc.2017.06.058
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发表时间：
2017-08
影响因子：
3.1
通讯作者：
A. Furuta;Toshinobu Nakamura
中科院分区：
文献类型：
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作者：
A. Furuta;Toshinobu Nakamura
在原始生殖细胞和着床前胚胎的两个发育阶段，DNA甲基化通过DNA去甲基化和去新甲基化的不同阶段进行动态重编程。在这里，我们发现核糖体DNA （rDNA）启动子在精子和卵母细胞中低甲基化；这种低甲基化在着床前发育期间得以维持。在胚胎第7.5天（E7.5）对胚胎和胚胎外细胞的DNA甲基化分析显示，rDNA启动子在胚胎和胚胎外区域轻度甲基化。有趣的是，这种低甲基化状态在E13.5和E18.5的生殖细胞发育过程中被观察到。相比之下，性腺和肝脏的胎儿体细胞在E13.5上获得甲基化，并在成人组织中保持。这些发现表明生殖细胞谱系中存在独特的rDNA甲基化特征。
DNA methylation is dynamically reprogrammed at two developmental periods, in primordial germ cells and pre-implantation embryos, via distinct phases of DNA demethylation andde novomethylation. Here we show that ribosomal DNA (rDNA) promoters are hypomethylated in sperm and oocytes; this hypomethyaltion was maintained during pre-implantation development. A DNA methylation analysis of embryonic and extra-embryonic cells on embryonic day 7.5 (E7.5) revealed that the rDNA promoter was slightly methylated in embryonic and extra-embryonic regions. Intriguingly, this hypomethylated status was observed throughout germ cell development on E13.5 and E18.5. In contrast, fetal somatic cells in gonad and liver acquired methylation on E13.5, which was maintained in adult tissues. These findings indicate a unique rDNA methylation signature in the germ cell lineage.