Characterization and expression analysis of a gene encoding CBF/DREB1 transcription factor from mangrove Aegiceras corniculatum
Characterization and expression analysis of a gene encoding CBF/DREB1 transcription factor from mangrove Aegiceras corniculatum
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DOI：
10.1007/s10646-015-1485-x
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发表时间：
2015-05
期刊：
影响因子：
2.7
通讯作者：
Ya-lan Peng;You-Shao Wang;Hao Cheng;Li-Ying Wang
中科院分区：
文献类型：
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作者：
Ya-lan Peng;You-Shao Wang;Hao Cheng;Li-Ying Wang
一些转录因子作为下游应激反应基因的主调控因子，在植物在寒冷、干旱和盐胁迫下的生存中发挥着重要作用。从红树青叶中分离到一个编码CBF/DREB1转录因子（C-repeat binding factor/脱水反应元件结合因子1）的基因，命名为AcCBF1。AcCBF1全长896 bp， ORF全长618 bp，编码205个氨基酸的蛋白。多序列分析表明，该蛋白与红树Avicennia marina的AmCBF1 （KC776908）具有100%的同源性，包含一个AP2/ERE dna结合域和两个CBF特征序列。实时荧光定量PCR的表达分析显示，正常情况下，AcCBF1基因在山菖蒲各组织中均有表达，其中在叶片中表达量最高。在非生物胁迫下，AcCBF1基因在不同组织中表现出不同的表达模式。一般来说，AcCBF1基因在寒冷和干旱条件下能被快速、强烈地诱导，而在脱落酸和盐度条件下则有轻微的诱导作用。此外，光可以正向调节AcCBF1的冷诱导水平。这些结果表明，AcCBF1可能在冷胁迫信号通路中发挥重要作用，并参与非生物胁迫间的串扰。进一步研究AcCBF1的启动子和下游应激响应基因，将有助于更好地理解红杉在非生物胁迫下的调控机制。
Several transcription factors play important roles in survival of plants under cold, drought and salt stresses by serving as master regulator of sets of downstream stress-responsive genes. A gene encoding CBF/DREB1 transcription factor (C-repeat binding factor/dehydration responsive element-binding factor 1) was isolated from mangrove Aegiceras corniculatum and designated AcCBF1. The full-length cDNA of AcCBF1 was 896 bp containing 618 bp ORF encoding a protein of 205 amino acids. Multiple sequence analysis showed that the corresponding protein had 100 % identity to AmCBF1 (KC776908) from mangrove Avicennia marina, and contains an AP2/ERE DNA-binding domain and two CBF signature sequences. Expression analyses based on quantitative real-time PCR revealed that the AcCBF1 gene was expressed in all tissues of A. corniculatum under normal condition with the highest expression level detected in leaves. When exposed to abiotic stresses, AcCBF1 gene showed different expression patterns in different tissues. Generally, AcCBF1 gene could be rapidly and strongly induced by cold and drought, while slightly induced by abscisic acid and salinity. Furthermore, light could positively regulate the cold-induction level of AcCBF1. These results suggest that the AcCBF1 may be playing important role in the signaling pathway of cold stress and also involved in the cross-talk among abiotic stresses. Further studies focusing on the promotors and downstream stress-responsive genes of AcCBF1 will help to better understand the regulatory mechanisms of mangrove A. corniculatum under abiotic stresses.