A COMPARISON OF THE EFFECT OF EPIDERMAL GROWTH-FACTOR, PLATELET-DERIVED GROWTH-FACTOR, AND FIBROBLAST GROWTH-FACTOR ON RAT PERIODONTAL-LIGAMENT FIBROBLAST-LIKE CELLS DNA-SYNTHESIS AND MORPHOLOGY
A COMPARISON OF THE EFFECT OF EPIDERMAL GROWTH-FACTOR, PLATELET-DERIVED GROWTH-FACTOR, AND FIBROBLAST GROWTH-FACTOR ON RAT PERIODONTAL-LIGAMENT FIBROBLAST-LIKE CELLS DNA-SYNTHESIS AND MORPHOLOGY
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DOI：
10.1902/jop.1994.65.5.373
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发表时间：
1994-05-01
影响因子：
4.3
通讯作者：
DABELSTEEN, E
中科院分区：
文献类型：
--
作者：
BLOM, S;HOLMSTRUP, P;DABELSTEEN, E
最近有报道称，在应用多肽生长因子后，牙周伤口的骨、牙本质和胶原纤维的形成增强。然而，复杂的体内环境使得无法确定生长因子的具体作用；不同细胞在伤口愈合过程中的作用。因此，我们研究了重组表皮生长因子（rEGF）、天然血小板衍生生长因子（nPDGF）和天然成纤维细胞生长因子（nFGF）对牙周韧带成纤维细胞样细胞的有丝分裂和形态发生作用。从大鼠PDL组织中建立细胞系。根据细胞形态、生长模式、细胞骨架蛋白和生长动力学对该细胞系进行了表征。通过在细胞DNA中掺入[H-3]胸苷4小时来评估生长因子的有丝分裂作用。各组间观察值的差异采用学生t检验进行评估。通过随机摄影记录，描述了生长因子在生长模式、细胞取向和细胞核形态方面的形态发生作用。通过存在被认为是正常成纤维细胞样细胞系特征的参数，已经确定了成纤维细胞样细胞类型和细胞系的非转化表型。实验和对照培养的形态发生分析显示，单层贴壁细胞具有纺锤形或星状形态，排列随机，细胞核圆形或细长。[H-3]-胸苷的掺入在所有生长因子的作用下呈剂量依赖性增加。对DNA合成的最大影响是：rEGF， 131%；nPDGF, 274%;nFGF为182%。结果表明，高浓度生长因子对PDL成纤维样细胞形态无明显影响。然而，当这些生长因子将来用于牙周治疗时，应考虑生长因子的有丝分裂效力的变化。
AN ENHANCED FORMATION OF BONE, DENTIN, AND COLLAGEN FIBERS in periodontal wounds after application of polypeptide growth factors has recently been reported. However, the complex environment in vivo makes it impossible to determine the specific effects of growth factors; on various cells involved in the wound-healing process. We have therefore investigated the mitogenic and morphogenic effects of recombinant epidermal growth factor (rEGF), natural platelet-derived growth factor (nPDGF), and natural fibroblast growth factor (nFGF) on periodontal ligament fibroblast-like cells. A cell line was established from rat PDL tissue. The cell line was characterized according to morphology, growth pattern, cytoskeletal proteins, and growth kinetics. The mitogenic effect of growth factors was assessed by incorporation of [H-3]thymidine in the cellular DNA for 4 hours. Differences between groups of observations were assessed by the Student t-test. The morphogenic effects of growth factors were described with respect to growth pattern, cell orientation, and cell and nucleus form after a random photographic recording. The fibroblast-like cell type and the non-transformed phenotype of the cell line have been identified by the presence of parameters considered to be characteristic of a normal fibroblast-like cell line. The morphogenic analysis of both experimental and control cultures showed a monolayer of adherent cells with spindle or stellate morphology, a random alignment and round or elongated nuclei. Incorporation of [H-3]-thymidine was increased in a dose-dependent manner by all growth factors. Maximal effect on the DNA synthesis was: rEGF, 131%; nPDGF, 274%; and nFGF, 182%. The results show that growth factors in high concentrations have no dramatic effects on the morphology of PDL fibroblast-like cells. However, variation in the mitogenic potency of the growth factors should be considered when these growth factors are used in periodontal treatments in the future.