Toll-like receptor expression in lupus peripheral blood mononuclear cells.
Toll-like receptor expression in lupus peripheral blood mononuclear cells.
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发表时间：
2007-03
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通讯作者：
K. Migita;T. Miyashita;Y. Maeda;Minoru Nakamura;H. Yatsuhashi;H. Kimura;H. Ishibashi;K. Eguchi
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作者：
K. Migita;T. Miyashita;Y. Maeda;Minoru Nakamura;H. Yatsuhashi;H. Kimura;H. Ishibashi;K. Eguchi
目的探讨Toll样受体（TLR）家族成员在系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中的表达。方法对14例SLE患者和15例健康人外周血单个核细胞（PBMC）进行分析。流式细胞术检测PBMC表面TLR2、TLR4的表达及胞内TLR9的表达。结果SLE患者外周血CD14+单核细胞表面TLR4的表达与正常对照组相比无显著性差异，但SLE患者外周血单核细胞表面TLR2的表达较正常对照组明显降低。SLE患者CD19 + B淋巴细胞胞内TLR9表达水平显著升高。然而，浆细胞样树突状细胞的TLR9表达水平在这些患者和健康受试者之间没有显著差异。结论SLE患者外周血B细胞表达TLR9，且表达增强。SLE患者B细胞TLR9表达上调可能与B细胞异常活化有关。
OBJECTIVE To investigate expression of members of the Toll-like receptor (TLR) family in peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE). METHODS We analyzed PBMC from 14 patients with SLE and 15 healthy subjects. The surface expressions of TLR2 and TLR4 and intracellular expression of TLR9 on PBMC were analyzed by flow cytometry. RESULTS Although TLR4 expressions on CD14+ monocytes were not significantly different between healthy subjects and patients with SLE, TLR2 expressions on monocytes were reduced in patients with SLE compared to healthy subjects. Intracellular TLR9 expression levels of CD19+ B lymphocytes were significantly elevated in patients with SLE. However, the TLR9 expression levels of plasmacytoid dendritic cells were not significantly different between these patients and healthy subjects. CONCLUSION Our results show that human peripheral blood B cells express TLR9 and that its expression is increased in patients with SLE. This upregulated expression of TLR9 in B cells may be related to the abnormal B cell hyperactivity in patients with SLE.