GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon.
GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon.
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GLABROUS (CmGL) 编码 HD-ZIP IV 转录因子，在甜瓜的多细胞毛状体起始中发挥作用。
DOI：
10.1007/s00122-017-3019-9
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发表时间：
2018
影响因子：
5.4
通讯作者：
Yang Luming
中科院分区：
文献类型：
--
作者：
Zhu Huayu;Sun Xiaofen;Zhang Qi;Song Pengyao;Hu Qianmei;Zhang Xiaojing;Li Xiang;Hu Jianbin;Pan Junsong;Sun Shouru;Weng Yiqun;Yang Luming
关键信息基于图谱的克隆鉴定出CmGL编码HD-ZIP IV型转录因子，控制甜瓜中多细胞毛状体的起始。 摘要毛状体是覆盖植物地上部分的小毛，源自表皮细胞，可以保护植物免受昆虫和病原体的损害。单细胞毛状体的调控途径已在模式植物拟南芥中得到了深入研究。对于具有多细胞毛状体的甜瓜（Cucumis meloL.）毛状体发育的遗传控制和调节知之甚少。在这项研究中，我们鉴定了一种甜瓜突变体 cmgl，其所有气生器官均完全无毛。进行了批量分离分析，以鉴定用于在具有 256 个 F2 植物的群体中进行连锁分析的多态性标记，这使得能够将 cmgll 位点定位在甜瓜染色体 VIII 中。在包含 1536 株 F2 植物的大型群体中，新一代测序辅助标记发现和精细定位将候选基因区域缩小至 12 kb，其中仅包含一个候选基因 cmgl，该基因编码 IV 类同源域相关亮氨酸拉链转录因子。在两个亲本系之间鉴定出 CmGL 基因编码区的四个 SNP；从 C 到 A 的单碱基替换导致了 cmgl 中的提前终止密码子和截短的蛋白质。 SNP 被转化为 dCAPS 标记，显示 F2 群体和 564 个甜瓜种质中的共分离。本研究结果将有助于更好地了解甜瓜毛状体发育的遗传控制以及开发新品种的标记辅助选择。
Key messageMap-based cloning identifiedCmGLthat encodes a HD-ZIP type IV transcription factor that controls multicellular trichome initiation in melon.AbstractTrichomes are small hairs covering the aerial parts of plants that originate from the epidermal cells, which can protect plants against the damage by insects and pathogens. The regulatory pathway of unicellular trichomes has been well studied in the model plantArabidopsis. Little is known about the genetic control and regulation of trichome development in melon (Cucumis meloL.) which has multicellular trichomes. In this study, we identified a melon mutant,cmgl, which showed completely glabrous on all aerial organs. A bulked segregant analysis was conducted to identify polymorphic markers for linkage analysis in a population with 256 F2plants, which allowed to locate thecmgllocus in melon chromosome VIII. Next-generation sequencing-aided marker discovery and fine mapping in a large population with 1536 F2plants narrowed the candidate gene region to 12 kb that harbored only one candidate gene forcmgl, which encoded a class IV homeodomain-associated leucine zipper transcription factor. Four SNPs in the coding region of theCmGLgene were identified between the two parental lines; a single base substitution from C to A resulted in a premature termination codon and a truncated protein in thecmgl. The SNP was converted into a dCAPS marker, which showed co-segregation in the F2population and 564 melon accessions. Result of this study will be helpful for better understanding of genetic control of trichome development in melon and marker-assisted selection in developing new cultivars.