Key metabolism pathways and regulatory mechanisms of high polysaccharide yielding in Hericium erinaceus.
Key metabolism pathways and regulatory mechanisms of high polysaccharide yielding in Hericium erinaceus.
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DOI：
10.1186/s12864-021-07480-x
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发表时间：
2021-03-06
期刊：
影响因子：
4.4
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Gong M;Zhang H;Wu D;Zhang Z;Zhang J;Bao D;Yang Y
猴头菇是一种名贵的食药用真菌，广泛应用于食品和医药领域。猴头菇多糖是主要的生物活性化合物，在医疗保健行业发挥着很高的生物活性价值。据报道，猴头菇原始菌株HEA基因组大小为38.16Mb，通过单分子实时测序技术编码9780个预测基因。系统发育分析表明，H. erinaceus 与 Dentipellis sp. 的进化亲缘关系最近。我们前期研究中通过ARTP诱变获得的突变菌株HEB和HEC的发酵菌丝体中多糖含量分别提高了23.25和47.45%，并且在HEC中产生了分子量为1.056 × 106 Da的新β-葡聚糖组分。转录组和蛋白质组学的综合分析表明，HEB和HEC中碳水化合物代谢途径模块的上调可能导致葡萄糖-6P的产生增加，并促进多糖重复单元的合成。 qPCR和PRM分析证实，大多数参与碳水化合物代谢的共富集和差异共表达基因与HEB和HEC中的转录组和蛋白质组数据具有相似的表达趋势。热图分析显示，HEC 中 RAS-cAMP-PKA 通路的蛋白表达谱显着降低，多糖含量大幅增加 47.45%。 S期进展阻断实验进一步验证了RAS-cAMP-PKA途径的功能障碍可能促进突变菌株HEC高多糖和β-葡聚糖的产生。该研究揭示了ARTP诱变诱导多糖合成增加的主要机制，并探索了多糖合成的关键基因和途径。在线版本包含可在 10.1186/s12864-021-07480-x 获取的补充材料。
Hericium erinaceus, a rare edible and medicine fungus, is widely used in the food and medical field. Polysaccharides from H. erinaceus are the main bioactive compound that exert high bioactive value in the medical and healthcare industries. The genome of H. erinaceus original strain HEA was reported 38.16 Mb, encoding 9780 predicted genes by single-molecule, real-time sequencing technology. The phylogenomic analysis showed that H. erinaceus had the closest evolutionary affinity with Dentipellis sp. The polysaccharide content in the fermented mycelia of mutated strains HEB and HEC, which obtained by ARTP mutagenesis in our previous study, was improved by 23.25 and 47.45%, and a new β-glucan fraction with molecular weight 1.056 × 106 Da was produced in HEC. Integrative analysis of transcriptome and proteomics showed the upregulation of the carbohydrate metabolism pathway modules in HEB and HEC might lead to the increased production of glucose-6P and promote the repeating units synthesis of polysaccharides. qPCR and PRM analysis confirmed that most of the co-enriched and differentially co-expressed genes involved in carbohydrate metabolism shared a similar expression trend with the transcriptome and proteome data in HEB and HEC. Heatmap analysis showed a noticeably decreased protein expression profile of the RAS-cAMP-PKA pathway in HEC with a highly increased 47.45% of polysaccharide content. The S phase progression blocking experiment further verified that the RAS-cAMP-PKA pathway’s dysfunction might promote high polysaccharide and β-glucan production in the mutant strain HEC. The study revealed the primary mechanism of the increased polysaccharide synthesis induced by ARTP mutagenesis and explored the essential genes and pathways of polysaccharide synthesis. The online version contains supplementary material available at 10.1186/s12864-021-07480-x.