A cycloheximide-enhanced protein in cytomegalovirus-infected cells.
A cycloheximide-enhanced protein in cytomegalovirus-infected cells.
复制标题
巨细胞病毒感染细胞中的放线菌酮增强蛋白。
DOI：
10.1016/0042-6822(80)90304-9
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发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
Gibson,W
中科院分区：
文献类型：
--
作者：
Jeang,KT;Gibson,W
在巨细胞病毒（CMV， Colburn菌株）感染细胞感染后早期用药物环己亚胺治疗后，一种其他次要蛋白质的合成量显著增加。基于变性聚丙烯酰胺凝胶的一维和二维分离的大小和电荷估计表明，该蛋白质的分子量为94K，净电荷为酸性。研究其合成的实验结果表明(i) 94K蛋白在感染后2-4小时内表达，（ii）其外观需要novoRNA合成，（iii）蛋白质及其mRNA似乎都是代谢稳定的，（iv）在环己亚胺抑制后该蛋白的数量增加似乎是mRNA选择性扩增的结果。根据94K蛋白在洗涤剂分离过程中的分配情况，认为94K蛋白可能在细胞质中起作用。
Following the treatment of cytomegalovirus (CMV, strain Colburn)-infected cells with the drug cycloheximide early after infection, dramatically enhanced amounts of an otherwise minor protein are synthesized. Size and charge estimates, based on one- and two-dimensional separations in denaturing polyacrylamide gels, indicate that this protein has a molecular weight of 94K and an acidic net charge. Results of experiments to investigate its synthesis indicate that (i) the 94K protein is expressed by 2–4 hr after infection, (ii) its appearance requiresde novoRNA synthesis, (iii) both the protein and its mRNA appear to be metabolically stable, and (iv) the increased amount of this protein following cycloheximide inhibition appears to be the result of selective mRNA amplification. On the basis of its partitioning during detergent fractionation procedures, it is suggested that the 94K protein may function in the cytoplasm.