Cell-type specific gene expression profiles of leukocytes in human peripheral blood.
Cell-type specific gene expression profiles of leukocytes in human peripheral blood.
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DOI：
10.1186/1471-2164-7-115
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发表时间：
2006-05-16
期刊：
影响因子：
4.4
通讯作者：
Brown, Patrick O
中科院分区：
文献类型：
--
作者：
Palmer, Chana;Diehn, Maximilian;Alizadeh, Ash A;Brown, Patrick O
血液是一个复杂的组织，由多种具有不同功能的细胞类型和相应的基因表达谱组成。我们试图确定血液中主要成分细胞的细胞类型特异性基因表达模式，包括B细胞、CD4+T细胞、CD8+T细胞、淋巴细胞和粒细胞。我们通过使用基因芯片比较纯化的B细胞、CD4+T细胞、CD8+T细胞、粒细胞和淋巴细胞的整体基因表达谱来做到这一点。非监督聚类分析表明，来自不同捐赠者的相似细胞群体拥有共同的基因表达谱。监督分析确定了B细胞(427个基因)、T细胞(222个基因)、CD8+T细胞(23个基因)、粒细胞(411个基因)和淋巴细胞(67个基因)的基因表达特征。没有发现有统计学意义的CD4+细胞的基因表达特征。在淋巴细胞亚群的不同基因中，编码细胞表面蛋白的基因不成比例地出现。淋巴细胞与粒细胞的区别在于其编码核糖体蛋白的基因表达水平较高，而粒细胞具有多种细胞表面蛋白和炎性蛋白的特征表达。构成细胞类型特定特征的基因包含了许多已知的参与细胞类型特定过程的基因，并提供了可能有助于发现许多仍未注释的基因的功能的线索。细胞类型特征基因最显著的特征是编码细胞表面蛋白的基因丰富，这可能反映了专门的环境感知系统对静止的白细胞生理的重要性。
Blood is a complex tissue comprising numerous cell types with distinct functions and corresponding gene expression profiles. We attempted to define the cell type specific gene expression patterns for the major constituent cells of blood, including B-cells, CD4+ T-cells, CD8+ T-cells, lymphocytes and granulocytes. We did this by comparing the global gene expression profiles of purified B-cells, CD4+ T-cells, CD8+ T-cells, granulocytes, and lymphocytes using cDNA microarrays. Unsupervised clustering analysis showed that similar cell populations from different donors share common gene expression profiles. Supervised analyses identified gene expression signatures for B-cells (427 genes), T-cells (222 genes), CD8+ T-cells (23 genes), granulocytes (411 genes), and lymphocytes (67 genes). No statistically significant gene expression signature was identified for CD4+ cells. Genes encoding cell surface proteins were disproportionately represented among the genes that distinguished among the lymphocyte subpopulations. Lymphocytes were distinguishable from granulocytes based on their higher levels of expression of genes encoding ribosomal proteins, while granulocytes exhibited characteristic expression of various cell surface and inflammatory proteins. The genes comprising the cell-type specific signatures encompassed many of the genes already known to be involved in cell-type specific processes, and provided clues that may prove useful in discovering the functions of many still unannotated genes. The most prominent feature of the cell type signature genes was the enrichment of genes encoding cell surface proteins, perhaps reflecting the importance of specialized systems for sensing the environment to the physiology of resting leukocytes.