THE EFFECT OF ORGAN-CULTURE ON HUMAN TRABECULAR MESHWORK
THE EFFECT OF ORGAN-CULTURE ON HUMAN TRABECULAR MESHWORK
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DOI：
10.1016/0014-4835(89)90080-8
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发表时间：
1989-07-01
影响因子：
3.4
通讯作者：
TSCHUMPER, RC
中科院分区：
文献类型：
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作者：
JOHNSON, DH;TSCHUMPER, RC
在一系列人眼中，利用灌流培养系统研究了器官培养对小梁网的影响。一对眼睛中的一只眼睛被培养，而另一只眼睛立即被戊二醛固定。培养周期从2天到28天不等，在此期间平均眼压为27毫米汞柱。维持15例。灌流的培养液似乎通过施莱姆的S管道和收集器通道离开眼睛，模拟体内的情况。在25只眼中，有21只眼的小梁网维持得很好。小梁板上的细胞保持在原位，细胞器通常保持正常，板层保持完好。培养诱导的变化被注意到，一些细胞形成胞浆内的脂空泡，另一些细胞形成肿胀的线粒体。此外，还可观察到散在的局灶性细胞坏死，最常见的是葡萄膜网。用图像分析系统测定网状细胞密度(核：实体组织)：与对照眼相比，培养眼细胞平均损失20%~40%。总体而言，器官培养似乎可以维持人类小梁网络至少28天，从而允许进行对照实验研究。
The effects of organ culture on the trabecular meshwork was studied in a series of human eyes using a perfusion culture system. One eye of a pair was cultured while the fellow eye was immediately fixed in glutaraldehyde. Culture periods ranged from 2 to 28 days, during which a mean intraocular pressure of 27 mmHg .+-. 15 was maintained. The perfused culture medium appeared to leave the eye via Schlemm''s canal and collector channels, mimicking the in vivo situation. The trabecular meshwork was well maintained with this system in 21 of 25 eyes. Cells remained in position on trabecular lamellae, cellular organelles usually remained normal, and the lamellae remained intact. Culture-induced changes were noted, with some cells developing intracytoplasmic lipid vacuoles and other cells developing swollen mitochondria. In addition, scattered focal cell necrosis was observed, most often in the uveal meshwork. Meshwork cellularity (nuclei:solid tissue) was determined with an image analysis system: there was an average cell loss of 20-40% in the cultured eyes as compared to their respective fellow control eyes. Overall, organ culture appears to maintain the human trabecular meshwork for at least 28 days, allowing controlled experimental studies to be performed.