Selective reduction of forskolin-stimulated cyclic AMP accumulation by inhibitors of protein synthesis.
Selective reduction of forskolin-stimulated cyclic AMP accumulation by inhibitors of protein synthesis.
复制标题
通过蛋白质合成抑制剂选择性减少毛喉素刺激的环 AMP 积累。
DOI：
10.1126/science.6190226
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Barovsky,K
中科院分区：
文献类型：
--
作者：
Brooker,G;Pedone,C;Barovsky,K
将C6-2B大鼠星形细胞瘤细胞与放线菌酮或艾美司汀孵育24小时，抑制蛋白质合成，当用100 μ M毛喉素攻击细胞30分钟时，细胞中3′，5 ′-单磷酸腺苷（cAMP）的积累逐渐减少。相比之下，霍乱毒素刺激（6 nM，3小时）环磷酸腺苷的积累并没有减少环己酰亚胺处理的细胞，和环磷酸腺苷只有轻微减少响应30分钟的挑战与10 μM（-）-异丙肾上腺素。这些实验表明存在以前未识别的环化酶组分，其对于毛喉素刺激的环AMP蓄积是必需的，并且具有比β-肾上腺素能受体、鸟嘌呤核苷酸调节蛋白或环化酶催化组分更短的半衰期。
Inhibiting protein synthesis by incubating C6-2B rat astrocytoma cells with cycloheximide or emetine for periods up to 24 hours caused a progressive decrease in the accumulation of adenosine 3′,5′-monophosphate (cyclic AMP) when the cells were challenged for 30 minutes with 100 μMforskolin. In contrast, cholera toxin-stimulated (6 nM, 3 hours) cyclic AMP accumulation was not diminished in cycloheximide-treated cells, and cyclic AMP was only minimally diminished in response to a 30-minute challenge with 10 μM(-)-isoproterenol. These experiments suggest the presence of a previously unrecognized cyclase component, which is essential for forskolin-stimulated cyclic AMP accumulation and has a shorter half-life than the β-adrenergic receptor, the guanine nucleotide regulatory proteins, or the cyclase catalytic component.