Chronic endometritis modifies decidualization in human endometrial stromal cells.
Chronic endometritis modifies decidualization in human endometrial stromal cells.
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DOI：
10.1186/s12958-017-0233-x
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发表时间：
2017-03-04
期刊：
影响因子：
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通讯作者：
Murakami T
中科院分区：
文献类型：
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作者：
Wu D;Kimura F;Zheng L;Ishida M;Niwa Y;Hirata K;Takebayashi A;Takashima A;Takahashi K;Kushima R;Zhang G;Murakami T
慢性子宫内膜炎(CE)是一种持续的子宫内膜炎症，通常无症状。由于行政长官一直被认为不会影响受影响妇女的生殖状况和整体健康，因此并未探讨其意义。然而，最近的研究表明，CE与体外受精-胚胎移植后反复着床失败、不明原因不孕症和反复流产有关。由于蜕膜分化为支持着床过程和维持妊娠，我们推测CE可能影响蜕膜形成过程。17例患者参与了子宫内膜间质细胞(ESCs)的培养。采集子宫内膜标本后，取胚胎干细胞培养13天。分别用ELISA法和Western blotting法检测培养上清液中催乳素(PRL)和胰岛素样生长因子结合蛋白-1(IGFBP-1)这两种已知的蜕膜化标志物在体外培养的ESCs中的浓度和蛋白表达，并计数细胞数。用实时定量聚合酶链式反应(RT-PCR)检测PRL和IGFBP-1的mRNA水平。由于性激素通过与性激素受体(ERα、ERβ、PRA和PRB)结合而诱导蜕膜的增殖和分化，因此对另外17例患者的石蜡包埋子宫内膜组织标本进行免疫组织化学检测和H评分半定量。与非CE组比较，CE组培养13天后，ESC细胞数增加，PRL和IGFBP-1α分泌减少。Western blotting检测CE患者ESCs中IGFBP-1蛋白表达的降低。逆转录聚合酶链式反应检测PRL和IGFBP-1mRNA表达降低。与非CE患者相比，CE患者间质细胞ERα、ERβ、PrA和pRb表达增加，而CE患者腺体细胞ERα和ERβ表达增加。我们的数据表明，CE通过不调谐性类固醇激素受体的功能来改变人胚胎干细胞的蜕膜化。本文的在线版本(doi：10.1186/s12958-0170233-x)包含补充材料，授权用户可以使用。
Chronic endometritis (CE) is a continuous inflammation of uterine endometrium, and it is usually symptomless. As CE has been thought not to affect the reproductive status and general health of affected women, its significance has not been explored. However, recent studies have shown that CE is related with repeated implantation failures after in vitro fertilization-embryo transfer, unexplained infertility, and recurrent miscarriages. As decidua differentiates to support the implantation process and maintains the pregnancy, we hypothesized that CE may influence the process of decidualization. Seventeen patients were employed in the experiment involving culture of endometrial stromal cells (ESCs). After obtaining endometrial samples, ESCs were harvested and cultured for 13 days. The concentrations in culture media and the protein expressions in ESCs of prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), two well known decidualization markers used in a large number of in vitro models, were analyzed by ELISA and Western blotting, respectively, and the cell numbers were also counted. The mRNA levels of PRL and IGFBP-1 were tested by quantitative real time polymerase chain reaction (RT-PCR). Since sex hormone induce proliferation and differentiation to decidua via binding to the sex hormone receptors (ERα, ERβ, PRA, and PRB), their expression was assessed in another 17 patients’ paraffin-embedded endometrial tissue specimens by immunohistochemistry and semi-quantified by H-score. Increased cell numbers and reduced secretion of PRL and IGFBP-1 were detected by ELISA in the ESCs of CE patients after culture for 13 days compared with non-CE patients. The decreased protein expression of IGFBP-1 in ESCs of CE patients was detected by Western blotting. The decreased expression of PRL mRNA and IGFBP-1 mRNA were detected by RT-PCR. Increased expressions of ERα, ERβ, PRA, and PRB were observed in the stromal cells of CE patients in comparison to non-CE patients, whereas increased expressions of ERα and ERβ were detected in the glandular cells of CE. Our data suggests that CE modifies decidualization of human ESC through untuning the function of sex steroid hormone receptor. The online version of this article (doi:10.1186/s12958-017-0233-x) contains supplementary material, which is available to authorized users.